Recent advances in three‐dimensional multicellular spheroid culture for biomedical research

By Ruei-Zeng Lin, Hwan-You Chang. Biotechnology Journal, 2008

1,251 citations recorded by the source corpus.

Subfields: Automotive Engineering, Biomedical Engineering, Oncology

View Paper: https://www.semanticscholar.org/paper/9e48b75c6f67959a1a413bdeea8a4dc8da1ce44a

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In Automotive Engineering

In Biomedical Engineering

In Oncology

Citations in the corpus

Multicellular tumor spheroids: an underestimated tool is catching up again. (2010, background): “Also, some new technologies such as digital deconvolution, multiphoton confocal microscopy, single-plane illumination microscopy or optical projection tomography may be useful for advanced spheroid imaging but should at present be exclusively considered for sophisticated analyses (Lin and Chang, 200”
Scaffold-Free Vascular Tissue Engineering Using Bioprinting (2009, methodology): “We first tested the new method with multicellular spheroids of distinct size (300 mm or 500 mm), which (compared to approaches for scaffold-free aggregation of cells into spheroids [20,25,37]) we are able to fabricate reliably and reproducibly.”
Is It Time to Start Transitioning From 2D to 3D Cell Culture? (2020, background): “3D melanoma spheroids are generated via the hanging drop method and provide researchers with the ability to study drug resistance within the cells (Lin and Chang, 2008).”
Opportunities and Challenges for use of Tumor Spheroids as Models to Test Drug Delivery and Efficacy (2012, neutral): “One valuable characteristic of spheroids is their diffusion limit of about 150–200 μm to many molecules, particularly oxygen [15].”
In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform (2016, background): “Overall, MCTS are more resistant to treatment than cells in 2D culture (Lin and Chang, 2008; Mehta et al., 2012; Fennema et al., 2013) and can recapitulate the drug resistance observed in solid tumors (Friedrich et al.”
Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors (2014, background): “Figure 3 shows the schematic diagram of typical zones of cell proliferation and the distribution of diffused oxygen, CO2, and nutrition in a 3D cell spheroid.(39) Such”
Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture (2018, background): “More broadly, several studies have illustrated that gene expression patterns are altered in 2D-monolayer cancer cell cultures, whereas results obtained from spheroids better recapitulate the molecular profiles of tumors in-vivo [21, 22].”
Three-Dimensional Cell Culture: A Breakthrough in Vivo (2015, background): “Compare to growing cells in 2D, 3D results in differential zones of proliferation due to oxygen, nutrient and waste gradients [25].”
Organ printing: Tissue spheroids as building blocks (2009, background): “3D robotic printer – ‘Fabber’ (designed by Cornell University, USA); c) 3D robotic industria Thus, facing the fact that most of the existing methods are not scalable [44], it is safe to predict that development of methods for scalable biofabrication of tissue spheroids will soon became a rapidly gro”