Multicellular tumor spheroids: an underestimated tool is catching up again.

By F. Hirschhaeuser, Heike Menne, C. Dittfeld, Jonathan West, W. Mueller‐Klieser, Leoni A. Kunz-Schughart. Journal of Biotechnology, 2010

1,595 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Cell Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/e0bfe42728249c27979567a81638caedb4f0d0e4

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Biomedical Engineering

1. Brendon M. Baker
2. Navid Kashaninejad
3. Christopher S. Chen
4. Stanley J. Osher
5. Nam-Trung Nguyen

In Cell Biology

1. Brendon M. Baker
2. Christopher S. Chen
3. Peter C. Searson

In Oncology

1. V. Silva-Vargas
2. Érika Pastrana
3. Peter C. Searson
4. F. Doetsch
5. Andrew D. Wong

Citations in the corpus

• Eyes Wide Open: A Critical Review of Sphere-Formation as an Assay For Stem Cells (2011, methodology): “Sphere-forming assays are increasingly used, both retrospectively and prospectively, to investigate stem cells and progenitors in many tissues during development and in the adult (Table 2), as well as in cancers and the cancer stem cell field (Hirschhaeuser et al., 2010; Clevers, 2011).”
• Spherical Cancer Models in Tumor Biology (2015, background): “However, the latter spheres have also been termed “ spheroids ” [40], “ colonospheres ” [41,42], and more recently “ colospheres ” [43,44].”
• Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture (2018, background): “This generates steep, physiologically relevant gradients, particularly in large spheroids [1,8,27,28] (Fig.”
• In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform (2016, methodology): “Studies comparing gene expression profiles of spheroids and 2D cultures to resected tumors revealed differences in genes associated with cell survival, proliferation, differentiation, and resistance to drug therapy and showed that spheroids more closely resembled in vivo tumors (Hirschhaeuser et al.”
• Opportunities and Challenges for use of Tumor Spheroids as Models to Test Drug Delivery and Efficacy (2012, neutral): “The peripheral cells closely reflect the in vivo environment of actively proliferating tumor cells next to capillaries, while the distant inner cells stay quiescent or die through necrosis and apoptosis [17].”
• Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues (2012, background): “For example, tumorcell spheroids of sufficient size possess a hypoxic necrotic core that is reminiscent of actual tumors, and have proved useful for studying the role of hypoxia in the production of tumorigenic factors and drug resistance (Hirschhaeuser et al., 2010; MuellerKlieser, 1997).”
• A review of level-set methods and some recent applications (2018, background): “one needs to consider clusters of at least a few thousands to a few tens of thousands cells [163, 164].”