Three-Dimensional Cell Culture: A Breakthrough in Vivo
By D. Antoni, H. Burckel, E. Josset, G. Noel. International Journal of Molecular Sciences, 2015
876 citations recorded by the source corpus.
Cell culture is an important tool for biological research. Two-dimensional cell culture has been used for some time now, but growing cells in flat layers on plastic surfaces does not accurately model the in vivo state. As compared to the two-dimensional case, the three-dimensional (3D) cell culture allows biological cells to grow or interact with their surroundings in all three dimensions thanks to an artificial environment. Cells grown in a 3D model have proven to be more physiologically relevant and showed improvements in several studies of biological mechanisms like: cell number monitoring, viability, morphology, proliferation, differentiation, response to stimuli, migration and invasion of tumor cells into surrounding tissues, angiogenesis stimulation and immune system evasion, drug metabolism, gene expression and protein synthesis, general cell function and in vivo relevance. 3D culture models succeed thanks to technological advances, including materials science, cell biology and bioreactor design.
Subfields: Automotive Engineering, Biomedical Engineering, Physiology
View Paper: https://www.semanticscholar.org/paper/5c853b4e3ffbc9ee8cb6b0ff4ce198f864c5f307
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Citations in the corpus
- Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources (2019, background): “Comparisons between 2D and 3D cell culture have revealed significant differences in proliferation, differentiation, drug toxicity resistance, gene expression and protein synthesis (Huyck et al., 2012; Antoni et al., 2015; Ravi et al., 2015; Cavo et al., 2016; Fang and Eglen, 2017; Riedl et al., 2017”
- Is It Time to Start Transitioning From 2D to 3D Cell Culture? (2020, methodology): “Furthermore, matrices often have multiple components that make them difficult to construct and require extensive amounts of labor (Antoni et al., 2015).”