Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells

By H. Niwa, J. Miyazaki, Austin G Smith. Nature Genetics, 2000

3,854 citations recorded by the source corpus.

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Citations in the corpus

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (2006, background): “Consistent with this idea, a mere 50% increase or decrease in Oct3/4 proteins induces differentiation of ES cells (Niwa et al., 2000). iPS clones overexpressed the four factors when RNA levels were analyzed,buttheirproteinlevels werecomparabletothosein…”
Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation. (2005, positive): “…(Tx)-inducible Cdx2, Cdx2ER, was introduced into ZHBTc4 ES cells, in which both of the endogenous Oct3/4 alleles are disrupted and a tetracycline (Tc)-regulable Oct3/4 transgene is expressed to maintain self-renewal (Niwa et al., 2000), resulting in the establishment of the ES cell line 4CER1.”
Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. (2003, background): “The dosage effect indicated for Nanog is also a feature of Oct-4 (Niwa et al., 2000) and Stat3 (Niwa et al.”
Oct4-induced pluripotency in adult neural stem cells. (2009, positive): “These studies of the developing embryo were complemented by studies of ESCs in which the Oct4 function was abolished (Niwa et al., 2000).”
An extended transcriptional network for pluripotency of embryonic stem cells. (2008, background): “Indeed, the dosage of Oct4 is crucial: reduced expression permit trophoectoderm development, whereas enhanced expression drives primitive endoderm differentiation (Niwa et al., 2000).”
The Polycomb Group Protein Suz12 Is Required for Embryonic Stem Cell Differentiation (2007, background): “In any event, it would be extremely interesting to know if the differentiation defects of Suz12 / ES cells depend on lack of Nanog and/or Oct4 repression; however, this experiment is unfortunately not feasible because ES cells do not grow in the absence of the two transcription factors (25, 29).”
Statistical confidence estimation for Hi-C data reveals regulatory chromatin contacts (2014, background): “10A), consistent with these genes’ roles as master regulators in undifferentiated ESCs regulating the maintenance of developmental potency (Niwa et al. 2000; Mitsui et al. 2003).”
HIF-2alpha regulates Oct-4: effects of hypoxia on stem cell function, embryonic development, and tumor growth. (2006, background): “As dose-dependent functions of Oct-4 in ES cells have previously been described (Scholer et al. 1990b; Niwa et al. 2000), we hypothesized that aberrant Oct-4 expression accounted for the low number of post-implantation Hif-2 KI/KI embryos recovered in our experiments, as well as their morphological ”
c-Myc regulates transcriptional pause release (2010, methodology): “For location analysis following Oct4 shutdown, ZHBTc4 mES cells (Niwa et al., 2000) were grown under standard mES cell culture conditions and expanded for two passages off MEF feeders.”
Strategies and new developments in the generation of patient-specific pluripotent stem cells. (2007, background): “Suppression of Oct-3/4 resulted in spontaneous differentiation into the trophoblast lineages in both mouse (Niwa et al., 2000) and human ES cells (Zaehres et al., 2005).”