Fractalkine Preferentially Mediates Arrest and Migration of CD16+ Monocytes

By P. Ancuța, R. Rao, A. Moses, Andrew Mehle, S. Shaw, F. Luscinskas, D. Gabuzda. Journal of Experimental Medicine, 2003

628 citations recorded by the source corpus.

CD16+ monocytes represent 5–10% of peripheral blood monocytes in normal individuals and are dramatically expanded in several pathological conditions including sepsis, human immunodeficiency virus 1 infection, and cancer. CD16+ monocytes produce high levels of proinflammatory cytokines and may represent dendritic cell precursors in vivo. The mechanisms that mediate the recruitment of CD16+ monocytes into tissues remain unknown. Here we investigate molecular mechanisms of CD16+ monocyte trafficking and show that migration of CD16+ and CD16− monocytes is mediated by distinct combinations of adhesion molecules and chemokine receptors. In contrast to CD16− monocytes, CD16+ monocytes expressed high CX3CR1 and CXCR4 but low CCR2 and CD62L levels and underwent efficient transendo-thelial migration in response to fractalkine (FKN; FKN/CX3CL1) and stromal-derived factor 1α (CXCL12) but not monocyte chemoattractant protein 1 (CCL2). CD16+ monocytes arrested on cell surface–expressed FKN under flow with higher frequency compared with CD16− monocytes. These results demonstrate that FKN preferentially mediates arrest and migration of CD16+ monocytes and suggest that recruitment of this proinflammatory monocyte subset to vessel walls via the CX3CR1-FKN pathway may contribute to vascular and tissue injury during pathological conditions.

Subfields: Immunology, Immunology and Allergy, Oncology

View Paper: https://www.semanticscholar.org/paper/92b7f93072ff8fb4ecddb90028dad40b17126fa0

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Citations in the corpus

Adhesion mechanisms regulating the migration of monocytes (2004, background): “fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=2214">CD16 + CCR2 − monocytes and has been proposed to be a resident cell population recruited to tissues independently of inflammatory stimul”
The CD14+ CD16+ blood monocytes: their role in infection and inflammation (2007, neutral): “[41] showed that the CD14 CD16 monocytes”
The neuropathogenesis of AIDS (2005, neutral): “; in contrast to CD14 + CD16 − monocytes, these cells express high levels of CX 3 C-chemokine receptor 1 (CX 3 CR1) and CXCR4, and they undergo transendothelial migration in response to CX 3 CL1 and CXCL12 (Ref”
Monocyte and macrophage differentiation: circulation inflammatory monocyte as biomarker for inflammatory diseases (2014, background): “In the same study, several groups reported differential expression of CD14 and CD14 within CD16 monocytes [26,30], which was related to distinct functional properties of the chemokine receptor expression pattern [31].”
The blood-brain barrier in brain homeostasis and neurological diseases. (2009, neutral): “…α ), CCL2 (MCP-1) and CX3CL1 (fractalkine): indeed, elevated levels of CXCL12 were detected in CSF and in brain tissue of HIV-infected patients [175,176], whereas high levels of the receptors of CXCL12 and CX3CL1 (CXCR4 and CX3CR1, respectively) were observed in HIV-infected monocytes [177,178].”
Monocyte and macrophage heterogeneity (2005, background): “For example, transendothelial migration of CD14 + CD16 + monocytes can be induced with soluble CX 3 C-chemokine ligand 1 (CX 3 CL1; also known as fractalkine) or CXC-chemokine ligand 12 (CXCL12; also known as SDF1α), the receptors for which are preferentially expressed by these cell”
Monocytes in atherosclerosis: subsets and functions (2010, background): “CX3CR1 has also been shown to be important for adhesion to the endothelium, and survival, of murine Gr1 − /Ly6C low monocytes and human CD16 + monocytes”
Immune cell migration in inflammation: present and future therapeutic targets (2005, background): “Macrophage subsets are less well defined than T cell subsets, although studies have identified a CCR2 hi CX3CR1 lo inflammatory subset and a CCR2 lo CX3CR1 hi homeostatic subse”
Human CD14dim Monocytes Patrol and Sense Nucleic Acids and Viruses via TLR7 and TLR8 Receptors (2010, neutral): “…cells better 382 Immunity 33, 375–386, September 24, 2010 ª2010 Elsevier Inc. than CD14+monocytes, a phenomenon in part dependent on the chemokine receptor CX3CR1 (Ancuta et al., 2003), and human CD14dimCD16+ monocytes patrol capillaries in vivo as shown in this report (see Figures 1H and 1I).”
Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases (2019, background): “explains the fact that they migrate and adhere more than CD16 monocytes to fractalkine-secreting endothelium (5, 25).”
Monocyte-mediated defense against microbial pathogens. (2008, background): “CD14+ monocytes express high levels of CCR1, CCR2, and CXCR2 and low levels of CX3CR1, whereas CD16+ monocytes express high levels of CX3CR1 and low levels of CCR2 (9,10).”