Identification of stem cells in small intestine and colon by marker gene Lgr5

By Nick Barker, J. V. Es, J. Kuipers, P. Kujala, M. V. D. Born, M. Cozijnsen, A. Haegebarth, J. Korving, H. Begthel, P. Peters and 1 more. Nature, 2007

5,667 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/9026ea96416a0a8d29b175f8ca7369d4413681fd

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. Hans Clevers
2. R. Nusse
3. Nick Barker
4. Elaine Fuchs
5. S. Morrison
6. C. Blanpain
7. A. Trumpp
8. Anne Wilson

In Molecular Biology

1. Hans Clevers
2. R. Nusse
3. Elaine Fuchs
4. S. Morrison
5. C. Blanpain
6. M. Clarke
7. Irving L. Weissman
8. Nick Barker

In Oncology

1. Hans Clevers
2. R. Nusse
3. S. Morrison
4. Nick Barker
5. Elaine Fuchs
6. C. Blanpain
7. M. Clarke
8. E. Batlle

Citations in the corpus

• Tracking adult stem cells (2011, background): “The combination of these properties is often referred to as stemness ; the minimal set of features that all stem cells have in common”
• Paneth cells, antimicrobial peptides and maintenance of intestinal homeostasis (2011, background): “1 ), and these stem cells remain the focus of much researc”
• The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse. (2011, methodology): “05) in Lgr5-GFP cells relative to Lgr5-GFP cells (Figures S2A–S2C) (Barker et al., 2007).”
• The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations (2011, methodology): “Intestinal epithelial cells were extracted from en bloc resected small intestine with 10 mM EDTA and manual shaking, followed by enzymatic dissociation with collagenase/dispase (Roche) to generate a single-cell suspension (1).”
• Single-cell dissection of transcriptional heterogeneity in human colon tumors (2011, methodology): “We analyzed and clustered colon epithelial cells using three genes encoding markers linked to either one of the two major cell lineages (that is, MUC2 for goblet cells and CA1 for enterocytes) or the immature compartment (that is, LGR5 ) of the colon epitheliu”
• Wnt/β-catenin signaling and disease. (2012, positive): “Labeled stem cells have been detected in organs ranging from the intestine to the stomach, the skin, the hair follicle, and the mammary gland (Barker et al., 2007; Barker et al., 2010; van Amerongen et al., 2012).”
• Wnt activity defines colon cancer stem cells and is regulated by the microenvironment (2010, background): “Moreover, microarray analysis on the highest and lowest 10% of TOP–GFP-expressing cells revealed that stem-cell-associated Wnt target genes, including those encoding the intestinal stem cell markers LGR5 (leucine-rich repeat-containing G protein-coupled receptor 5) and ASCL2 (achaete-scute-like 2) (”
• Transcription factor achaete scute-like 2 controls intestinal stem cell fate. (2009, methodology): “Protocols for in vitro transcription and in situ hybridizations are described elsewhere (Gregorieff et al., 2005). b-galactosidase (LacZ) staining was done as described previously (Barker et al., 2007).”
• Gremlin 1 Identifies a Skeletal Stem Cell with Bone, Cartilage, and Reticular Stromal Potential (2015, background): “After 6 to 9 months, intestinal Grem1 cells had expanded to give rise to the mesenchymal sheath subjacent to the Lgr5+ crypt base columnar stem cell zone (Figures 6B, 6C, and 6E) (Barker et al., 2007).”