Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

By K. Okita, M. Nakagawa, Hong Hyenjong, T. Ichisaka, S. Yamanaka. 2008

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Citations in the corpus

Induced pluripotency: history, mechanisms, and applications. (2010, methodology): “The first integration-free iPSCs were generated from adult mouse hepatocytes using nonintegrating adenoviral vectors (Stadtfeld et al. 2008c), and from mouse embryonic fibroblasts (MEFs) transfected with plasmids (Okita et al. 2008).”
A fresh look at iPS cells. (2009, methodology): “In an independent study, our group generated iPS cells from mouse embryonic fibroblasts using plasmids (Okita et al., 2008).”
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon (2009, positive): “However, their efficiencies were 100~1000-fold lower than those achieved by retroviral reprogramming 10, 11.”
Klf4 reverts developmentally programmed restriction of ground state pluripotency (2009, background): “low copy number integration and Cre-mediated excision facilitated formal proof that once attained, the Epi-iPS cell state does not require the continuous presence of introduced DNA elements (Okita et al., 2008; Stadtfeld et al., 2008).”
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. (2011, neutral): “We used three iPSC lines, 20D17 (Okita et al., 2007), 178B-5 (Nakagawa et al., 2008), and 492B-4 (Okita et al., 2008), all bearing Nanog-EGFP (NG) transgenes .”
Hypoxia enhances the generation of induced pluripotent stem cells. (2009, methodology): “Generation of iPS cells with plasmid transfection was performed as previously described (Okita et al., 2008).”
Reprogramming of mouse and human cells to pluripotency using mature microRNAs. (2011, background): “Most of the reprogramming methods reported so far involve introduction of genetic materials and thus run the risk that exogenous vector sequences can be integrated into the host genome (Okita et al., 2008; Kaji et al., 2009; Woltjen et al., 2009; Jia et al., 2010).”
Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “Furthermore, the generation of genetically unmodified iPSCs argues that insertional mutagenesis is not an essential step in the process (Okita et al., 2008; Stadtfeld et al., 2008).”
Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors (2011, methodology): “Toward this end, several techniques have been used for obtaining integration and/or transgene-free iPSCs, including the use of plasmids (3, 4), the Cre/ loxP system (5, 6), adenoviruses (7), piggyBac (8, 9), minicircle vector (10), and proteins (11, 12).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, methodology): “The first integration-free iPS cells were generated from mouse hepatocytes using adenoviral vectors (Stadtfeld et al., 2008b), and from MEFs by plasmid transfection (Okita et al., 2008).”