Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues

By J. Hanna, Krishanu Saha, R. Jaenisch. Cell, 2010

746 citations recorded by the source corpus.

Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell state. In addition, evidence has accumulated that distinct states of pluripotency can inter-convert through the modulation of both cell-intrinsic and exogenous factors. To fully realize the potential of in vitro reprogrammed cells, we need to understand the molecular and epigenetic determinants that convert one cell type into another. Here we review recent advances in this rapidly moving field and emphasize unresolved and controversial questions.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/fdadccd279e46ec41527950a4b14fd011955a6c1

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Citations in the corpus

A census of human RNA-binding proteins (2014, result): “Accordingly, we investigated the mRNA expression of 1,441 RBPs and 1,463 TFs profiled in a microarray study that measured transcript levels of 16,867 protein‑coding genes across 31 human tissues96, assuming that transcript abundance approximates pro‑ tein abundance in the cell97,98.”
The promise of induced pluripotent stem cells in research and therapy (2012, methodology): “Regardless of the method of derivation, iPS cells maintain the key features of ES cells, including the ability to propagate in culture indefinitely and the capacity to generate cells from each of the three embryonic germ layers (see ref”
Reconstructing and reprogramming the tumor propagating potential of glioblastoma stem-like cells (2014, positive): “…decisions can be overridden by artificial induction of combinations of ‘core’ TFs that yield induced pluripotent stem (iPS) cells or direct lineage conversion (Hanna et al., 2010; Morris and Daley, 2013; Orkin and Hochedlinger, 2011; Takahashi and Yamanaka, 2006; Vierbuchen and Wernig, 2011).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “In this regard, it has been suggested that the pluripotent state can be classified in two different ways: ICM-like or “naïve” state, and epiblast-like or “primed” state (Hanna et al., 2010; Nichols and Smith, 2009).”
Control of Embryonic Stem Cell State (2011, background): “, 2008), the influence of extrinsic factors on pluripotency (Pera and Tam, 2010), and cellular reprogramming into ESC-like states (Hanna et al., 2010; Stadtfeld and Hochedlinger, 2010; Yamanaka and Blau, 2010).”
Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions (2014, positive): “…the exact function of TET proteins in mouse ESCs and embryonic development, recent studies support a role for TET enzymes in transcription factor (Oct4/Klf4/Sox2/c-Myc, referred to as OKSM)-mediated reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (Hanna et al., 2010).”
Discovery of cell-type specific DNA motif grammar in cis-regulatory elements using random Forest (2018, neutral): “, fibroblasts to pluripotent cells) [10].”
Discovery of non-directional and directional pioneer transcription factors by modeling DNase profile magnitude and shape (2014, neutral): “” Nature biotechnology 32 (2): 171-178.”
Single-cell expression analyses during cellular reprogramming reveal an early stochastic and a late hierarchic phase. (2012, background): “, 2010) induce reprogramming rapidly and possibly as a single event with little heterogeneity observed in somatic cells, possibly consistent with a deterministic process (Hanna et al., 2010).”