Klf4 reverts developmentally programmed restriction of ground state pluripotency

By G. Guo, Jian Yang, Jennifer Nichols, J. S. Hall, Isobel Eyres, William Mansfield, Austin G Smith. Development, 2009

812 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/c5892890d95e14c1f39d9b2c9467c54422b8a8aa

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In Molecular Biology

Citations in the corpus

Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “…into a primed EpiSC-like state in vitro by promoting the signaling of TGF-b, Activin, and FGF2, EpiSCs can epigenetically revert back to naive pluripotency by a variety of genetic manipulations and culture conditions (Figure 2) (Bao et al., 2009; Guo et al., 2009; Hanna et al., 2009a).”
Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs (2010, background): “X-chromosome inactivation represents an important epigenetic difference between the two states of pluripotency: Whereas female mESCs are in a pre-X inactivation state with two active X chromosomes (XaXa), mEpiSCs and most if not all hESCs have already undergone X inactivation (XiXa) (10, 28).”
A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. (2010, background): “Figure S3-related to Figure 3 a, qPCR for the indicated genes in 2 iPSC clones compared to MEFs. b, Scatter plot representation of DNA microarrays comparing the gene expression profiles of R1 ESCs, MEFs, and an iPSC cell line.”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “For example, epiblast-derived stem cells (EpiSCs) (Brons et al. 2007; Tesar et al. 2007) have been isolated from post-implantation embryos, embryonic germ cells (EGCs) (Matsui et al. 1992; Resnick et al. 1992) have been derived from primordial germ cells (PGCs) of the midgestation embryo, and multip”
Nanog Is the Gateway to the Pluripotent Ground State (2009, positive): “Nanog / pre-iPS cells were transfected using Nucleofection with 1 mg of PBfloxNanogPgkHygro plus 2–3 mg PBase expression vector, pCAGPBase (Guo et al., 2009), to obtain cells that express constitutively Nanog and Hygromycin resistance.”
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. (2011, neutral): “5) epiblasts (Guo et al., 2009; Han et al., 2010).”
Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo (2009, background): “The presence of two active X chromosomes is a hallmark of the pluripotent ground state of ES cells that is not shared by epiblast stem cells (EpiSCs), extra-embryonic trophectoderm stem (TS) or extra-embryonic endoderm (XEN) cell lines (Guo et al., 2009).”
Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human (2014, background): “…are expressed at very low levels or are absent from EpiSC and human PSC. Strikingly, however, transfection of EpiSC with a single component in conjunction with transfer to 2iL can ignite the entire circuitry and reset the ESC ground state (Guo et al., 2009; Hanna et al., 2009; Silva et al., 2009).”
Systematic discovery of Xist RNA binding proteins (2015, methodology): “We converted ESC into EpiSC in the presence of Fgf2 and Activin (Guo et al., 2009).”
Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014, positive): “EpiSCs can be converted to naive pluripotency by combined chemical and genetic manipulation (Guo et al., 2009; Hanna et al., 2009; Silva et al., 2009).”
Naive and primed pluripotent states. (2009, methodology): “ESCs can be induced to differentiate into EpiSCs by exposure to activin and Fgf, but the reverse transition requires transfection with Klf4 or other reprogramming factors.”