Efficient initiation of HCV RNA replication in cell culture.

By K. Blight, A. Kolykhalov, Charles M. Rice. Science, 2000

1,529 citations recorded by the source corpus.

Subfields: Hepatology, Molecular Biology, Virology

View Paper: https://www.semanticscholar.org/paper/8c8c8c82068c3ee6230067a1cf5821e8fa31999e

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Citations in the corpus

A Quantitative High-Resolution Genetic Profile Rapidly Identifies Sequence Determinants of Hepatitis C Viral Fitness and Drug Sensitivity (2014, background): “The establishment of the HCV replicon cell [10,11] and infectious [12–14] systems have paved the way for highthroughput screening of small-molecule inhibitors, and thereby aided the identification of many new classes of antiviral compounds [15,16].”
Identification of the Hepatitis C Virus RNA Replication Complex in Huh-7 Cells Harboring Subgenomic Replicons (2003, background): “The recent development of replicon systems for HCV has allowed, for the first time, the study of efficient and genuine HCV RNA replication in Huh-7 human hepatoma cells in vitro (6, 13, 19, 25).”
Unravelling hepatitis C virus replication from genome to function (2005, background): “The next discovery was that replicon RNAs harboured culture-adaptive mutations, often in NS5A, that increased RNA replication and Neo-transduction efficiency by up to 10,000-fol”
Viruses and interferon: a fight for supremacy (2002, background): “This limitation has been overcome partially by the development of the HCV replicon, which is an autonomously replicating subgenomic viral RNA that encodes a drug-selectable marker and the viral components that are required for authentic HCV RNA replicatio”
Antiviral Actions of Interferons (2001, background): “A deletion of 47 amino acids encompassing the ISDR of NS5A is among the adaptive mutations clustered in NS5A that confer an increased efficiency of HCV RNA replication in culture; this and other adaptive HCV mutants were IFN sensitive (41).”
Flaviviridae :T he Viruses and Their Replication (2007, background): “The correlation between ISDR sequence change and IFN resistance, however, has not yet been confirmed in other studies (547), and this region of NS5A was shown to be dispensible for HCV RNA replication in cell culture (59,416).”
Immunology of hepatitis B virus and hepatitis C virus infection (2005, methodology): “For in vitro studies of HCV biology, several experimental systems have now become available that allow analysis of HCV replication and polyprotein processin”