Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

By Shiaoching Gong, M. Doughty, C. R. Harbaugh, A. Cummins, M. Hatten, N. Heintz, Charles R. Gerfen. Journal of Neuroscience, 2007

909 citations recorded by the source corpus.

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificial chromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5) produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

Subfields: Cell Biology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8664743227adaa31f799ef292b54b77bccbce28b

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Citations in the corpus

Recombinase-driver rat lines: tools, techniques, and optogenetic application to dopamine-mediated reinforcement (2011, positive): “Here, we explore an alternative approach addressing all of the above fundamental limitations, instead generating a panel of the first characterized and specific transgenic recombinase-driver rat lines, with regulatory information contained in 200–300 kb of DNA upstream and downstream of the target g”
Dopaminergic modulation of synaptic transmission in cortex and striatum (2012, background): “…in transgenic mice using bacterial artificial chromosomes (BACs) that express Cre recombinase or fluorescent proteins such as enhanced green fluorescent protein (EGFP) or tdTomato under control of the promoter region for D1 or D2 receptor genes (Ade et al., 2011; Gong et al., 2003, 2007).”
Modulation of striatal projection systems by dopamine (2011, neutral): “This point has been driven home more recently by the development of BAC transgenics in which eGFP or Cre-recombinase are expressed under control of the D1 or D2 promoter (Gong et al., 2003; 2007).”
Whole-brain mapping of direct inputs to midbrain dopamine neurons. (2012, positive): “To resolve these methodological issues, we combined the Cre/loxP gene expression system (Gong et al., 2007) with rabies-virus-based transsynaptic retrograde tracing (Wickersham et al., 2007b) to comprehensively identify monosynaptic inputs to a genetically defined neural population (Haubensak et…”
The Neuroanatomical Organization of the Basal Ganglia (2010, background): “Recent studies in which PT corticostriatal neurons have been identified in BAC-Cre transgenic lines suggest that these neurons are widely distributed in somatosensory, sensorimotor, and prefrontal cortical areas with collaterals projecting to each of the subcortical targets (Gerfen et al., 2013; Gon”
Opposing Patterns of Signaling Activation in Dopamine D1 and D2 Receptor-Expressing Striatal Neurons in Response to Cocaine and Haloperidol (2008, background): “Our findings confirm and extend previous assessments of drd1a-EGFP and drd2EGFP mice (Gong et al., 2003, 2007; Lee et al., 2006; Lobo et al., 2006).”
Genetic dissection of neural circuits. (2008, neutral): “Indeed, Cre drivers are being created as NIH-sponsored projects (e.g., http://www.mmrrc.org; http://www.gensat. org) (Gong et al., 2007).”
A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping (2008, background): “these cell types (Gong et al., 2007).”
A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing (2012, background): “Hundreds of Cre mouse lines, generated in individual labs and through large-scale efforts 8–10 , have been established to direct specific gene expression or deletion in a wide range of cell types or populations throughout the nervous system.”