A FLEX Switch Targets Channelrhodopsin-2 to Multiple Cell Types for Imaging and Long-Range Circuit Mapping

By Deniz Atasoy, Yexica Aponte, H. Su, Scott Sternson. Journal of Neuroscience, 2008

638 citations recorded by the source corpus.

### Introduction Genetic approaches have yielded progress toward characterizing the relationship of molecularly defined neuronal cell types to behavior. However, relatively little is known about the functional properties of the connections between these molecularly defined cell types. This

Subfields: Cellular and Molecular Neuroscience, Cognitive Neuroscience

View Paper: https://www.semanticscholar.org/paper/b4d310d1fbb9a2fe590e3599bf6b8f31c7f7d44f

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Citations in the corpus

The Development and Application of Optogenetics (2011, background): “This doublefloxed inverted open-reading-frame (DIO; reviewed in Zhang et al. 2010) strategy (Atasoy et al. 2008, Sohal et al. 2009, Tsai et al. 2009) situates the opsin gene (inhibitory or excitatory) in the inverted (meaningless) orientation, but the gene is flanked by two sets of incompatible Cre…”
Rapid, reversible activation of AgRP neurons drives feeding behavior in mice. (2011, methodology): “Stable transgene inversion was achieved using the FLEX Switch (12).”
Neuron-type specific signals for reward and punishment in the ventral tegmental area (2012, methodology): “We confined expression to dopaminergic neurons by injecting adeno-associated virus containing FLEX-ChR2 (AAV-FLEX-ChR2”
Sexually dimorphic neurons in the ventromedial hypothalamus govern mating in both sexes and aggression in males (2013, positive): “Moreover, we establish that a discrete, sexually dimorphic neuronal population influences sexually dimorphic behaviors in both sexes.”
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain (2009, background): “Cre-responding recombinant viral vectors can confer strong expression, and they offer a fast, convenient alternative approac”
Dopamine reward prediction errors reflect hidden state inference across time (2017, positive): “In the first surgery, we injected 500nL of adeno-associated virus (AAV, serotype 5) carrying an inverted ChR2 (H134R) fused to the fluorescent reporter eYFP and flanked by double loxP sites 3,37 .”
Whole-brain mapping of direct inputs to midbrain dopamine neurons. (2012, positive): “Production of viral vectors Different serotypes of AAVs and different promoters were used to express RG and TVA under the control of a FLEX switch (Atasoy et al., 2008), to avoid any possible competition.”
AGRP neurons are sufficient to orchestrate feeding behavior rapidly and without training (2010, methodology): “, fused to the fluorophore tdtomato (ChR2:tdtomato), was targeted to AGRP or POMC neurons by injecting the Cre recombinase–dependent viral vector rAAV-FLEX- rev -ChR2:tdtomat”
Driving opposing behaviors with ensembles of piriform neurons. (2011, methodology): “In a second set of experiments, a ChR2 gene in the reverse orientation was flanked by pairs of loxP sites, such that expression of ChR2 was dependent upon Cre recombinase (Atasoy et al., 2008).”
Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures (2010, background): “To amplify the transcriptional activity in a cell-specific manner, conditional AAV vector”