Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans

By J. Pak, Andrew Fire. Science, 2007

674 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/81e2af9f3952ed7b474665559fdadca2c27505c4

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In Molecular Biology

Citations in the corpus

piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans (2012, background): “RNAi effects are generally systemic (soma and germline) and are observed in the F1 generation, but the latter requires the generation of secondary siRNAs (Grishok et al., 2000; Pak and Fire, 2007; Sijen et al., 2007; Gu et al., 2009).”
Origins and Mechanisms of miRNAs and siRNAs (2009, background): “In at least some cases, the role of Dicer in silencing extends beyond dsRNA processing and into the pathway of RISC assembly, as discussed in more detail below, and this activity is much more dependent on the dsRBD partner protein.”
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. (2011, background): “…through a cellular RNA-dependent RNA polymerase (RdRP) that amplifies 168 the effect of gene silencing by transitive generation of target gene-derived secondary siRNAs 169 induced by the injected or orally administered dsRNA (Sijen et al., 2001, Pak and Fire, 2007; 170 Sijen et al., 2007).”
Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide. (2008, background): “C. elegans has 27 Argonaute proteins that must distinguish between primary and secondary siRNAs bearing monophosphate and triphosphate termini, respectively (Pak and Fire, 2007; Sijen et al., 2007; Yigit et al., 2006).”
Antiviral immunity directed by small RNAs. (2007, neutral): “…secondary siRNAs are exclusively antisense , have 5" di-or triphosphates, and are thought to occur as individual RNA-dependent RNA polymerase products, or " copy " (c)RNA, directly synthesized from target mRNA upon primary siRNA-guided cleavage (Pak and Fire, 2007; Sijen et al., 2007; Figure 2B).”
Human tRNA-derived small RNAs in the global regulation of RNA silencing. (2010, background): “Secondary siRNAs in RNAi of Caenorhabditis elegans are 59-triphosphorylated (Pak and Fire 2007)—apparently, the result of short transcripts produced by an RNA-dependent RNA polymerase (RdRP)—and are loaded into specialized members of the Argonaute family (Aoki et al. 2007).”
Transgenerational Epigenetic Inheritance: Prevalence, Mechanisms, and Implications for the Study of Heredity and Evolution (2009, positive): “This replication is a twostage process, the details of which differ between taxa, and it leads to the amplification of siRNAs that act as repressors of gene expression (Baulcombe 2007; Pak and Fire 2007).”
PRG-1 and 21U-RNAs interact to form the piRNA complex required for fertility in C. elegans. (2008, result): “NAs could be explained by a two-step model similar to one previously described for RDE-1-directed silencing in C. elegans (Yigit et al., 2006; Sijen et al., 2007; Pak and Fire, 2007 ).”
Specialization and evolution of endogenous small RNA pathways (2007, background): “Recent, surprising results indicate that secondary siRNAs are probably manufactured exclusively during the RdRP reaction, without the need for subsequent dicing of dsRN”