RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
By O. Terenius, A. Papanicolaou, Jennie S. Garbutt, I. Eleftherianos, H. Huvenne, S. Kanginakudru, M. Albrechtsen, C. An, J. Aymeric, Andrea Barthel and 63 more. Journal of insect physiology, 2011
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Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.
Subfields: Molecular Biology
View Paper: https://www.semanticscholar.org/paper/23618414347649b7a9803e2d0ae7ca2206505360
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Citations in the corpus
- Literature review of baseline information on RNAi to support the environmental risk assessment of RNAi‐based GM plants (2018, background): “Another observation casting doubt over the link between sil gene repertoire and efficient environmental or systemic RNAi, was the fact that several lepidopteran species, which are much less sensitive to (systemic) RNAi (Terenius et al., 2011), also contain three sil genes, including B.”