Multiplex PCR: Optimization and Application in Diagnostic Virology

By Elfath M. Elnifro, A. Ashshi, R. Cooper, P. Klapper. Clinical Microbiology Reviews, 2000

919 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/810c4192086e6cc68c3f1e1361778699ecc29d9f

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Citations in the corpus

Multiplex polymerase chain reaction: A practical approach (2002, background): “Efforts to enhance sensitivity and specificity, and to facilitate automation have resulted in numerous publications regarding the application of multiplex PCR in the diagnosis of infectious agents, especially those which target viral nucleic acids (21).”
Nutritional evaluation of cookies produced from pigeon pea, cocoyam and sorghum flour blends (2011, methodology): “, (1988), this method has been applied in many areas of DNA testing, including species identification, analyses of deletions, mutations, polymorphism, quantitative assays, and reverse transcription PCR (Elnifro et al., 2000; Subbotin and Moens, 2006).”
Real-time PCR in the microbiology laboratory. (2004, background): “Multiplex PCR uses one or more primer sets to potentially amplify multiple templates within a single reaction [128,129].”
Current trends in microsatellite genotyping (2011, background): “Although several review articles on multiplex development already exist (Edwards & Gibbs 1994; Henegariu et al. 1997; Elnifro et al. 2000; Markoulatos et al. 2002; Wallin et al. 2002; Butler 2005a; Cryer et al. 2005), none of these papers has provided a complete overview of SSR identification, multi”
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. (2009, background): “The ability to multiplex greatly expands the power of qPCR analysis ( 65 , 66), particularly when applied to thesimultaneousdetectionofpointmutationsorpolymorphisms (67).”
Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, background): “Alternatively, multiplex PCR using multiple primer sets in the same reaction can decrease specimen and reagent consumption but is limited in detection sensitivity due to uneven amplification efficiencies of the different primer sets (Elnifro et al. , 2000).”
PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings (2004, positive): “91, 92 With ever-increasing evidence supporting the prognostic value of identifying drug-resistant mutations, routine genotypic resistance testing is now standard care in the treatment of HIV-infected patients.”