PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
By Samuel Yang, R. Rothman. Lancet. Infectious Diseases (Print), 2004
929 citations recorded by the source corpus.
Summary Molecular diagnostics are revolutionising the clinical practice of infectious disease. Their effects will be significant in acute-care settings where timely and accurate diagnostic tools are critical for patient treatment decisions and outcomes. PCR is the most well-developed molecular technique up to now, and has a wide range of already fulfilled, and potential, clinical applications, including specific or broad-spectrum pathogen detection, evaluation of emerging novel infections, surveillance, early detection of biothreat agents, and antimicrobial resistance profiling. PCR-based methods may also be cost effective relative to traditional testing procedures. Further advancement of technology is needed to improve automation, optimise detection sensitivity and specificity, and expand the capacity to detect multiple targets simultaneously (multiplexing). This review provides an up-to-date look at the general principles, diagnostic value, and limitations of the most current PCR-based platforms as they evolve from bench to bedside.
Subfields: Clinical Biochemistry, Epidemiology, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/e22763953040cb9f458d5e021d46ad2981d22c0a
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Citations in the corpus
- A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything (2017, methodology): “The multiplexing option is essential for detection and quantification in diagnostic qPCR assays that rely on the inclusion of internal amplification controls (Yang and Rothman, 2004; Kubista et al., 2006; Bustin et al., 2009).”
- Point-of-care diagnostics for global health. (2008, background): “Although the PCR platforms are ruggedized and adapted for field operation, they were inadequate in their ability to concentrate and purify nucleic acid at low concentration in large volumes of water.”
- Advances in Microfluidic PCR for Point-of-Care Infectious Disease Diagnostics (2011, methodology): “…particularly in detecting difficult-to-culture, limb- or life-threatening, biothreat or emerging infectious pathogens, have demonstrated significant performance and cost advantages over traditional methods (Rothman et al. , 2010, Yang et al. , 2008a, Yang and Rothman, 2004, Yang et al. , 2008b).”