The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

By Stephen A. Bustin, Vladimir Benes, J. Garson, Jan Hellemans, J. Huggett, M. Kubista, Reinhold D. Mueller, T. Nolan, M. W. Pfaffl, G. Shipley and 2 more. Clinical Chemistry, 2009

15,160 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f8531552e2e55395057ab73a24cffe28f6f9ad0c

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Citations in the corpus

Aquaporins: Highly Regulated Channels Controlling Plant Water Relations1 (2014, methodology): “…mRNA abundance is nowadays measured by reverse transcriptionquantitative PCR approaches, a widely used technique that however requires a strict design of the experimental conditions (primer specificity and efficiency, housekeeping genes for normalization, and analysis method; Bustin et al., 2009).”
Cell-free nucleic acids as biomarkers in cancer patients (2011, methodology): “The development of PCR-based assay standardization is needed in order to report clinical and prognostic biomarkerresults that are similar to those outlined in the recent minimal information for the publication of quantitative real-time PCR guideline”
Reporting Guidelines to Increase the Reproducibility and Comparability of Research on Microplastics (2020, positive): “Other fields, like molecular biology, 34 proteomics, 35 and transcriptomics, 36 already have highly successful examples of reporting guidelines that have been widely adopted by their field, and we hope this work serves a similar purpose in our field.”
Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products (2017, methodology): “For in-house validation of the proposed real-time PCR normalised system, different parameters were assessed, namely trueness, precision, repeatability and robustness (Bustin et al., 2009; ENGL, 2015).”
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020. (2020, methodology): “dPCR requires the same careful assay design considerations as those required for qPCR (53, 54).”
Linking N2O emissions from biochar-amended soil to the structure and function of the N-cycling microbial community (2013, methodology): “During qPCR setup, evaluation and data analysis, we followed the MIQE guidelines (Bustin et al., 2009).”
Reference genes in real-time PCR (2013, background): “It is relentlessly stressed that many qPCR experiments lack authors critical evaluation, are wrongly designed and difficult to repeat due to insufficient data quality (Bustin et al. 2009).”