Multiplex polymerase chain reaction: A practical approach
By P. Markoulatos, N. Siafakas, M. Moncany. Journal of clinical laboratory analysis (Print), 2002
643 citations recorded by the source corpus.
Subfields: Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/f0fc217e5f4a8c187e0151d718e0ca73d9a45cdc
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Genetics
- Alan R. Lemmon
- E. Lemmon
- R. Petit
- M. Mccarthy
- S. Wagner
- Olivier Lepais
- Thibaut Malausa
- Phillip A. Morin
In Molecular Biology
- Alan R. Lemmon
- E. Lemmon
- R. Petit
- M. Mccarthy
- S. Wagner
- Olivier Lepais
- Thibaut Malausa
- Phillip A. Morin
Citations in the corpus
- High-Throughput Genomic Data in Systematics and Phylogenetics (2013, background): “…PCR include (a) off-target amplification as a consequence of combining many primers from different loci in the same reaction, (b) formation of an excess of primer dimers, (c) uneven or no amplification of some target loci, and (d ) low amplification repeatability (Markoulatos et al. 2002).”
- Highly accurate SNP genotyping from historical and low‐quality samples (2007, background): “Recent advances in multiplex PCR methodologies now appear to make screening of nuclear genetic variation possible for the first time from a wide range of poor-quality samples (Lindblad-Toh et al . 2000; Markoulatos et al . 2002, 2003; Shapero et al . 2004; Krause et al . 2006; Rompler et al . 2006”
- Current trends in microsatellite genotyping (2011, background): “Although several review articles on multiplex development already exist (Edwards & Gibbs 1994; Henegariu et al. 1997; Elnifro et al. 2000; Markoulatos et al. 2002; Wallin et al. 2002; Butler 2005a; Cryer et al. 2005), none of these papers has provided a complete overview of SSR identification, multi”
- Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations (2015, background): “…factors for a successful mPCR assay include the PCR buffer concentrations, the balance between magnesium chloride and deoxynucleotide concentrations, the quantities of DNA template, cycling temperatures and Taq DNA polymerase (Markoulatos et al., 2002; Cheah et al., 2008; Khoo et al., 2009).”