Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements

By F. J. Mojica, Chcsar Díez-Villaseñor, Jesús García-Martínez, E. Soria. Journal of Molecular Evolution, 2005

2,036 citations recorded by the source corpus.

Subfields: Ecology, Insect Science, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7e34b66fe25dae4b19d28b6d6dec2b4246845547

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

1. Rodolphe Barrangou
2. Feng Zhang
3. L. Marraffini
4. E. Charpentier
5. J. van der Oost
6. Philippe Horvath
7. P. Hsu
8. Sylvain Moineau

In Insect Science

1. Rodolphe Barrangou
2. Feng Zhang
3. E. Charpentier
4. Yong‐Bi Fu

In Molecular Biology

1. Rodolphe Barrangou
2. Feng Zhang
3. Jennifer A. Doudna
4. L. Marraffini
5. E. Charpentier
6. P. Hsu
7. J. van der Oost
8. Eric S. Lander

Citations in the corpus

• Short motif sequences determine the targets of the prokaryotic CRISPR defence system. (2009, methodology): “In the case of E. coli, spacer sequences additional to those from complete genomes were obtained from E. coli Reference (ECOR) collection strains (Ochman & Selander, 1984) after PCR amplification and sequencing of CRISPR loci as previously described (Mojica et al., 2005).”
• CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity (2014, background): “Their biological role remained elusive until 2005, when three groups reported that the spacers were homologous to foreign genetic elements, including viruses and plasmids (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
• The CRISPR/Cas9 system for plant genome editing and beyond. (2015, background): “, 1987), their biological function was not understood until 2005, when it was shown that the spacers were homologous to viral and plasmid sequences suggesting a role in adaptive immunity (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
• A Guild of 45 CRISPR-Associated (Cas) Protein Families and Multiple CRISPR/Cas Subtypes Exist in Prokaryotic Genomes (2005, background): “The guild is presumed to be involved in processes that may include the maintenance of repeat clusters [3], capture of new spacer elements [12,13] and expansion or contraction of clusters, propagation of the leader sequence and repeat clusters within a genome [3,7], transfer of CRISPR and cas genes t”
• Rethinking Protein Drug Design with Highly Accurate Structure Prediction of Anti-CRISPR Proteins (2021, background): “Using this performance as a basis, we further examined the Acr proteins without experimental structures with AlphaFold2, to predict the structural diversity of these genetically distinct proteins that are natural inhibitory proteins against prokaryotic CRISPR-Cas systems.”
• The Biology of CRISPR-Cas: Backward and Forward. (2018, positive): “A milestone in CRISPR research was the realization that the spacer sequences match plasmids and phage genomes, as it was the first hint that CRISPR-Cas might function as a prokaryotic defense mechanism (Bolotin et al., 2005; Mojica et al., 2005; Pourcel et al., 2005).”
• CRISPR-Cas12a: Functional overview and applications (2020, background): “mation between the environment and prokaryotic genomes [12].”
• Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilus (2007, background): “We thank Denise Tremblay, Simon Labrie, and Julie Samson for stimulating discussions; Yanick Bourbeau for the DNA display conception; and Gene Bourgeau for editorial assistance.”
• CRISPR-Cas systems in bacteria and archaea: versatile small RNAs for adaptive defense and regulation. (2011, background): “The year 2005 marked a turning point when three groups independently reported that the hypervariable spacers showed sequence homology to viruses (or bacteriophages) or plasmids and hypothesized that CRISPRs and associated proteins could play a role in immunity against transmissible genetic elements ”