Short motif sequences determine the targets of the prokaryotic CRISPR defence system.

By F. J. Mojica, C. Díez-Villaseñor, Jesús García-Martínez, C. Almendros. Microbiology, 2009

1,201 citations recorded by the source corpus.

Subfields: Cardiology and Cardiovascular Medicine, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/34a9705afbaaeae9f26dadf5fe505c23fd3e1569

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In Cardiology and Cardiovascular Medicine

In Molecular Biology

Citations in the corpus

Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli (2012, background): “The observed PAM was AWG, in accordance with the reported motif (16) as analyzed by Weblogo (22) (Figure 2B).”
Reprogramming homing endonuclease specificity through computational design and directed evolution (2013, positive): “For example, the CRISPR/Cas9 family of nucleases is becoming a popular catalyst for genome engineering (6,49–51), and the methods presented in this work could be used to increase the speciﬁcity of Cas9 (52–54) or expand the targeting potential of this class of enzymes, currently limited by the PAM s”
The Biology of CRISPR-Cas: Backward and Forward. (2018, neutral): “In type I and type II systems, the adaptation machinery selects protospacers with a PAM that is compatible with the interference machinery (Datsenko et al., 2012; Deveau et al., 2008; Mojica et al., 2009; Swarts et al., 2012).”
Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, methodology): “…allow CRISPR-Cas immune systems to differentiate between self (the CRISPR array spacer) and non-self (the invader DNA) and have been heavily implicated in spacer acquisition (Deveau et al., 2008; Heler et al., 2015; Horvath et al., 2008; Marraffini and Sontheimer, 2010; Mojica et al., 2009).”
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence (2017, background): “…which results in the initiation of the cleavage of the crRNA–foreign DNA hybridization complex, mediating survival of bacteria after viral infections while protecting themselves from lysis (Brouns et al., 2008; Mojica et al., 2009; Deveau et al., 2010; Garneau et al., 2010; Bhaya et al., 2011).”
RNA-guided DNA insertion with CRISPR-associated transposases (2019, methodology): “Given that CRISPR-Cas effectors require a protospacer adjacent motif (PAM) to recognize target DNA (29), we generated a target plasmid (pTarget) library containing the PSP1 sequence flanked by a 6N motif upstream of the protospacer.”
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea (2010, background): “The alignment of protospacer flanking sequences in phage genomes reveals the presence of short (2–3 nt) conserved regions that have been named 'CRISPR motifs' or 'protospacer-adjacent motifs' on either side of the protospacer sequenc”
CRISPR-Cas systems: prokaryotes upgrade to adaptive immunity (2014, background): “The PAM and seed requirements for cleavage are readily exploited by mutant phages containing modifications of either of these sequences, which prevent cleavage and CRISPR immunity (Barrangou et al., 2007; Deveau et al., 2008).”