Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilus

By H. Deveau, Rodolphe Barrangou, Josiane E. Garneau, J. Labonté, C. Fremaux, Patrick Boyaval, D. Romero, Philippe Horvath, Sylvain Moineau. Journal of Bacteriology, 2007

1,227 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/971c3eade11406cd614dcd3076c0873c9de77a1b

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Citations in the corpus

Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, background): “The sgRNA often contains a unique 20 base-pair (bp) sequence designed to complement the target DNA site in a sequence-specific manner, and this must be followed by a short DNA sequence upstream essential for the compatibility with the Cas9 protein used, which is termed the “protospacer adjacent moti”
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria (2012, background): “Single point mutations in the PAM or defined protospacer positions allow the phages or plasmids to circumvent CRISPR-mediated immunity (16, 17, 20).”
Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus (2007, methodology): “periments were carried out according to protocols previously outlined (2, 7).”
Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli (2012, background): “It was suggested from DNA sequence analyses, and later shown experimentally, that short, 2–5 bp sequences found near the protospacer, called protospacer adjacent motifs (PAMs), are crucial for the interference step *To whom correspondence should be addressed.”
The new frontier of genome engineering with CRISPR-Cas9 (2014, neutral): “Theprotospacer adjacent motif (PAM), a short sequence motif adjacent to the crRNA-targeted sequence on the invading DNA, plays an essential role in the stages of adaptation and interference in type I and type II systems ( 39 , 56 – 58 ).”
Development and Applications of CRISPR-Cas9 for Genome Engineering (2014, neutral): “…that protospaceradjacent motifs (PAMs) may direct the type II Cas9 nuclease to cleave DNA (Bolotin et al., 2005), Moineau and colleagues highlighted the importance of PAM sequences by demonstrating that PAM mutations in phage genomes circumvented CRISPR interference (Deveau et al., 2008).”
CRISPR interference (CRISPRi) for sequence-specific control of gene expression (2013, background): “The targeting specificity is determined both by base pairing between the RNA chimera and the target DNA and by the binding between the Cas9 protein and a short DNA motif commonly found at the 3 ′ end of the target DNA, called the protospacer adjacent motif (PAM) 12,15,16 .”