Differences between human and mouse embryonic stem cells.

By I. Ginis, Yongquan Luo, T. Miura, S. Thies, R. Brandenberger, S. Gerecht-Nir, M. Amit, Ahmet Hoke, M. Carpenter, J. Itskovitz‐Eldor and 1 more. Developmental Biology, 2004

731 citations recorded by the source corpus.

We compared gene expression profiles of mouse and human ES cells by immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis. Several markers that in concert could distinguish undifferentiated ES cells from their differentiated progeny were identified. These included known markers such as SSEA antigens, OCT3/4, SOX-2, REX-1 and TERT, as well as additional markers such as UTF-1, TRF1, TRF2, connexin43, and connexin45, FGFR-4, ABCG-2, and Glut-1. A set of negative markers that confirm the absence of differentiation was also developed. These include genes characteristic of trophoectoderm, markers of germ layers, and of more specialized progenitor cells. While the expression of many of the markers was similar in mouse and human cells, significant differences were found in the expression of vimentin, beta-III tubulin, alpha-fetoprotein, eomesodermin, HEB, ARNT, and FoxD3 as well as in the expression of the LIF receptor complex LIFR/IL6ST (gp130). Profound differences in cell cycle regulation, control of apoptosis, and cytokine expression were uncovered using focused microarrays. The profile of gene expression observed in H1 cells was similar to that of two other human ES cell lines tested (line I-6 and clonal line-H9.2) and to feeder-free subclones of H1, H7, and H9, indicating that the observed differences between human and mouse ES cells were species-specific rather than arising from differences in culture conditions.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7d16dda3296b2b3b87740248393d4178fca02e3c

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Citations in the corpus

Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts (2008, neutral): “Mouse and human ES cells have a distinct expression pattern of cell surface markers that characterize the undifferentiated state (Ginis et al., 2004).”
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative (2007, background): “The available data also suggest differences between human and mouse ES (mES) cell”
Embryonic stem cell differentiation: emergence of a new era in biology and medicine. (2005, background): “To identify the developing germ cells in the differentiation cultures, the ES cells carried a modified Oct4 promoter that drove GFP expression specifically in the germline.”
Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells (2005, background): “The hypothesis of Nodal-mediated epiblast pluripotency potentially reconciles certain compelling differences between mouse and human ESCs (Ginis et al., 2004).”
Embryonic stem cells: prospects for developmental biology and cell therapy. (2005, background): “These included the presence of transcripts to Oct-3/4, Nanog, Tdgf1, Utf1, and lin-28 in undifferentiated hES cells, but remarkably, Sox2, Dnmt3B, gp130 and Rex-1 (ZFP42) were inconsistently or poorly expressed among several lines (31, 103, 138).”
Specification of motoneurons from human embryonic stem cells (2005, result): “and are corroborated by a recent comparison of gene expression profiles in mouse and human ES cell”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, background): “In addition, expression of MDM proteins, the negative regulators of p53, may also contribute to the inactivation of p53 in mES cells (Ginis et al., 2004).”