Real-time DNA sequencing from single polymerase molecules.

By J. Korlach, K. Bjornson, Bidhan Chaudhuri, R. Cicero, B. Flusberg, J. Gray, David Holden, R. Saxena, Jeffrey Wegener, S. Turner. Methods in Enzymology, 2010

1,801 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7c912da9680d9c68203fb867dd2445e05c00d93b

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Citations in the corpus

SNP Discovery through Next-Generation Sequencing and Its Applications (2012, background): “A laser placed below the ZMW excites only the ﬂuorophores of the incorporated nucleotides as the ZMW entraps the light and does not allow it to reach the unincorporated nucleotides above [32].”
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies (2012, methodology): “In silico evaluation of primer pairs suitable for sequencing technologies such as PacBio SMRT or classical clone libraries (Group L) For fragments >1000 bases we could not find an archaeal primer pair with both an overall coverage of over 70% and a satisfying phylum spectrum (Supplementary Table S21”
Sequencing technologies and genome sequencing (2011, background): “Subsequently, the labeled nucleotide incorporation and detection allow us to determine the DNA sequence (Levene et al. 2003; Eid et al. 2009).”
Generations of Sequencing Technologies: From First to Next Generation (2017, methodology): “There are two main approaches that characterize TGS [22]: The single molecule real time sequencing approach (SMRT) [38] that was developed by Quake laboratory [41-43] and the synthetic approach that rely on existing short reads technologies used by Illumina (Moleculo) [43] and 10xGenomics (https://w”
Third generation sequencing: technology and its potential impact on evolutionary biodiversity research (2016, methodology): “Sequencers distributed by Pacific Biosystems (PacBio) use Single Molecule Real-Time (SMRT) sequencing (Eid et al., 2009), whereas Oxford Nanopore Techniques (ONT) developed a device for nanopore sequencing (Branton et al., 2008).”
A Primer on Metagenomics (2010, background): “sequencing that will enable the sequencing of a single chromosome in a single pass with few or no fragments should be established very soon [35,36].”
Assembly Algorithms for Next-Generation Sequencing Data (2010, neutral): “Pacific Biosciences machines [9] might produce reads longer than first-generation machines.”
Mind the Gap: Upgrading Genomes with Pacific Biosciences RS Long-Read Sequencing Technology (2012, background): “A side effect of sequencing double-stranded DNA templates [18] with a high error-rate is that occasionally the hairpin adapters on the ends of the template are not properly identified and removed.”
Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations (2018, background): “…(PacBio), which rely on detection of fluorescent nucleotide incorporation by single, immobilized polymerases within zero-mode wave guides 89 , and those of Oxford Nanopore Technologies, which capitalize on the differential current changes caused by nucleotides as a single-stranded DNA…”
The IPD and IMGT/HLA database: allele variant databases (2014, methodology): “The analysis of the data produced by NGS techniques, whether it is the long reads associated with the Pacific Biosciences SMRT technology (9,10) or shorter tiled reads associated with Roche 454 (11), Illumina (12) and ION-PGM, Ion-Torrent (13), still require an accurate reference database in order t”
Development of genome-wide SNP assays for rice (2010, background): “Third-generation platforms use single-molecule sequencing without the requirement for DNA amplification, and they produce relatively long reads (>1 kb) in real time (Eid et al. 2009).”
Next‐generation sequencing technologies for environmental DNA research (2012, methodology): “The single-molecule real-time (SMRT) DNA-sequencing platform, introduced in 2010 by Pacific Biosciences, is another example of a real-time, fluorescence-based, SMS platform (Korlach et al. 2010).”