Generations of Sequencing Technologies: From First to Next Generation
By Mehdi Kchouk, Jean-François Gibrat, Mourad Elloumi. 2017
164 citations recorded by the source corpus.
DNA sequencing process utilizes biochemical methods in order to determine the correct order of nucleotide bases in a DNA macromolecule using sequencing machines. Ten years ago, sequencing was based on a single type of sequencing that is Sanger sequencing. In 2005, Next Generation Sequencing Technologies emerged and changed the view of the analysis and understanding of living beings. Over the last decade, considerable progress has been made on new sequencing machines. In this paper, we present a non-exhaustive overview of the sequencing technologies by beginning with the first methods history used by the commonly used NGS platforms until today. Our goal is to provide beginners in the field as well as to the amateurs of science a simple and understandable description of NGS technologies in order to provide them with basic knowledge as an initiation into this field in full ardor.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/a016143b0cddd6a47722f062e719de3f1062cbec
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Molecular Biology
In Plant Science
Citations in the corpus
- Microbial Biodiversity and Bioremediation Assessment Through Omics Approaches (2020, methodology): “The pyrosequencing technique is sequencing by synthesis approach based on the detection of pyrophosphate release after the addition of a nucleotide to the newly synthesized DNA strand (Mardis, 2008; Kchouk et al., 2017).”
- Artificial intelligence (AI) and big data in cancer and precision oncology (2020, background): “This device is capable of single-molecule sequencing minus library preparation at lower costs and reduced time without compromising quality by generating longer reads [11,14].”