Structural basis for 5′-nucleotide base-specific recognition of guide RNA by human AGO2

By F. Frank, Nahum Sonenberg, B. Nagar. Nature, 2010

626 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/60e4bbd0fa5081251d2685dcd6aa038f0f335732

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

In Molecular Biology

Citations in the corpus

Exosome and Exosomal MicroRNA: Trafficking, Sorting, and Function (2015, background): “The AGO2 protein in humans, which prefers to bind to U or A at the 50 end of miRNAs, plays an important role in mediating mRNA:miRNA formation and the consequent translational repression or degradation of the mRNA molecule [63].”
Small RNA sorting: matchmaking for Argonautes (2010, background): “Recently, our understanding of small RNA-sorting determinants has expanded substantially, and Argonaute and RNA structural studies have begun to provide a mechanistic basis for observations from in vitro and in vivo analyse”
Metazoan MicroRNAs (2018, background): “This pocket prefers a 5′-terminal pU or pA (Frank et al., 2010; Suzuki et al., 2015) as well as the 5′-nucleoside monophosphate of the strand with the least stable 5′ terminal pairing (Khvorova et al., 2003; Schwarz et al., 2003).”
Chemistry, structure and function of approved oligonucleotide therapeutics (2023, background): “The interaction between the 5′-phosphate and a strongly positively polarized binding pocket on the MID domain (332) is probably key to loading and discrimination between guide and passenger strand.”
The Structure of Human Argonaute-2 in Complex with miR-20a (2012, neutral): “Their 50 ends occupy a pocket within the Argonaute Mid domain, which may confer 50 end nucleotide preferences upon some Ago family members (Boland et al., 2010; Frank et al., 2010; Ma et al., 2005; Mi et al., 2008; Parker et al., 2005).”
microRNAs in action: biogenesis, function and regulation (2023, background): “However, as the miRNA 5′ base is bound by the specificity loop in the Ago MID domain 55,56 , it is not actually available for pairing 19,20 .”
The Crystal Structure of Human Argonaute2 (2012, background): “3A), as seen in the structure of the isolated MID domain (18).”
Molecular mechanisms of RNA interference (2013, neutral): “The electrostatic environment generated by the functional groups of this loop is incompatible with CMP or GMP binding, a result consistent with NMR titration experiments showing that binding affinities of UMP (0.12 mM) and AMP (0.26 mM) are 30-fold lower than those measured for either CMP (3.6 mM) o”
The widespread regulation of microRNA biogenesis, function and decay (2010, background): “In addition, identity of the 5′-terminal nucleotide may also directly affect the efficiency of miRNA loading into miRISC, independent of the duplex end stabilit”