High mobility of proteins in the mammalian cell nucleus

By R. Phair, Tom Misteli. Nature, 2000

1,257 citations recorded by the source corpus.

Subfields: Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5f7c0c6a15fe1bea6e5f29ca7a7141f99fef2417

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

In Molecular Biology

Citations in the corpus

Kinetics of Core Histones in Living Human Cells (2001, methodology): “This approach has been used with H2A, H2B, and the linker histone H1 (Kanda et al., 1998; Dey et al., 2000, Lever et al., 2000, Misteli et al., 2000; Perche et al., 2000; Phair and Misteli, 2000).”
Kinetic modelling approaches to in vivo imaging (2001, neutral): “For example, the core histone H3 is virtually immobile, whereas a similarly sized splicing factor is highly mobil”
Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light (2006, background): “1 ) similar to those of the corresponding enhanced (E)GFP-tagged protein”
From silencing to gene expression: real-time analysis in single cells. (2004, background): “…may provide insight not only into transcriptional regula(Cheutin et al., 2003; Festenstein et al., 2003) in contrast tion but possibly also into chromatin structure. to the core histones, which show little turnover (Kimura and Cook, 2001; Lever et al., 2000; Phair and Misteli, 2000).”
Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles (2015, background): “Fluorescence recovery after photobleaching (FRAP) experiments interrogating organelles such as the nucleolus and Cajal bodies indicate that their constituent molecules internally diffuse rapidly (Phair and Misteli, 2000), and P-granules, the worm analog of mammalian nuage or germ granules, condense ”
Analysis of binding reactions by fluorescence recovery after photobleaching. (2004, background): “Indeed most recent FRAP experiments seek to infer something about how the GFP-tagged protein interacts with binding sites within the cell (e.g., McNally et al., 2000; Phair and Misteli, 2000).”
An extensive network of coupling among gene expression machines (2002, background): “, and the movement of SR proteins within the nucleus is rapid and ATP-independen”
Studying protein dynamics in living cells (2001, background): “More sophisticated equations and modelling are also required for analysing FRAP experiments in which there are several diffusing species or when recovery occurs by more than one process (for example, when diffusion and binding/release from a substrate occur”
Dynamics of proteins in solution (2019, background): “Numerous studies have addressed protein diffusion in living cells (Lippincott-Schwartz et al., 2001), in the nucleoplasm (Phair and Misteli, 2000), in the mitochondrial lumen (Partikian et al., 1998), and in the cytoplasm (Wojcieszyn et al., 1981; Swaminathan et al., 1997; Arrio-Dupont et al.,…”