Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light

By N. Gurskaya, V. Verkhusha, A. S. Shcheglov, D. Staroverov, T. Chepurnykh, A. Fradkov, Sergey A. Lukyanov, Konstantin A. Lukyanov. Nature Biotechnology, 2006

724 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/a577f30be4dd8ce33fcfaaaacb185de0d7686b3c

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Citations in the corpus

SLP‐2 is required for stress‐induced mitochondrial hyperfusion (2009, neutral): “…was accompanied by fusion of the inner mitochondrial membrane and continuity of the matrix lumen of fused mitochondria, we ﬁrst expressed Dendra2, a photoactivable probe, which is photoconverted from green to red on brief excitation with UV, in mitochondria of WT MEFs (Gurskaya et al , 2006).”
Fluorescent probes for super-resolution imaging in living cells (2008, background): “In this regard, Dendra-2, with similar contrast and brightness, could potentially outperform monomeric Eos, because it matures properly at 37°C and can be activated by blue light (which causes less damage to tissue than the ultraviolet (UV) light that is required for monomeric Eos”
Fluorescent proteins and their applications in imaging living cells and tissues. (2010, background): “To overcome this limitation, significant efforts were devoted to develop monomeric Kaede-like proteins, resulting in a series of markers (136, 141, 281, 312, 478), the best of which are currently mEos2 (281) and Dendra2 (3, 136) (see table in Fig.”
Guidelines for the use and interpretation of assays for monitoring autophagy (2012, background): “Dendra2 is an improved version of the green-to-red photoswitchable fluorescent protein Dendra, which is derived from the octocoral Dendronephthya sp.(184) Dendra2 is capable of irreversible photoconversion from a green to a red fluorescent form, but can be used also as a normal GFP or RFP vector.”
The dendritic branch is the preferred integrative unit for protein synthesis-dependent LTP (2011, methodology): “, 1991), and Dendra (Gurskaya et al., 2006) was sparsely introduced via biolistic gene transfection.”
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (2012, positive): “Dendra2 is an improved version of the green-to-red photoswitchable fluorescent protein Dendra, which is derived from the octocoral Dendronephthya sp.(267) Dendra2 is capable of irreversible photoconversion from a green to a red fluorescent form, but can be used also as a normal GFP or RFP vector.”
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging (2011, positive): “Examples in the second category include dark-to-bright fluorescent proteins, such as PA-GFP 28 and PA-mCherry 29 , color-converting fluorescent proteins, such as PS-CFP 30 , EosFP 31,32 , and Dendra2 33 , and push-pull fluorogen dyes 18 , although in some cases photoactivatable fluorescent proteins ”