Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons.

By T. Kitamoto. 2001

889 citations recorded by the source corpus.

Subfields: Aging, Cellular and Molecular Neuroscience, Genetics

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Citations in the corpus

Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014, methodology): “We first screened 27 MBON split-GAL4 driver lines, crossed to a multi-insert UAS-Shibirets1 effector line (UAS-Shits1 on the third chromosome) (Kitamoto, 2001) at 34°C. 3–7 days old adult males of each genotype were wet starved for 1–4 hr and then were assayed for 22 parameters of basic locomotion…”
Dopamine Is Required for Learning and Forgetting in Drosophila (2012, positive): “We drove expression of a UAS-shi transgene encoding a temperature-sensitive Dynamin protein that blocks synaptic output at restrictive temperatures (Kitamoto, 2001) or a UAS-trpA1 transgene encoding a temperature-sensitive cation channel to stimulate DANs at elevated temperatures (Hamada et al.”
System-like consolidation of olfactory memories in Drosophila (2013, neutral): “DOI:10.1523/JNEUROSCI.0451-13.2013 Copyright © 2013 the authors 0270-6474/13/339846-09$15.00/0 copies on the third chromosome (Kitamoto, 2001), (2) uas-G-CaMP3.”
Rapid Consolidation to a radish and Protein Synthesis-Dependent Long-Term Memory after Single-Session Appetitive Olfactory Conditioning in Drosophila (2008, methodology): “For these experiments, we used neuron-specific expression of the dominant temperaturesensitive shibire ts1 (shi ts1) transgene (Kitamoto, 2001). shi ts1 blocks dynamin-dependent membrane recycling and thereby synaptic vesicle release at the restrictive temperature of 31°C, and this blockade is…”
Limited taste discrimination in Drosophila (2010, background): “Taste conditioning experiments were performed on flies in which the output of theMBs was conditionally blocked, usingMBGal4 lines to express temperature-sensitive shibire, a dominantnegative dynamin [UAS-shi(ts)] (23).”
Different Kenyon Cell Populations Drive Learned Approach and Avoidance in Drosophila (2013, methodology): “…This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.”
Transposition Driven Genomic Heterogeneity in the Drosophila Brain: A Dissertation (2012, positive): “Using uas-shibirets1 (Kitamoto, 2001) as a temperature-sensitive blocker of DPM contribute to the memory process.”
Parallel Pathways for Cross-Modal Memory Retrieval in Drosophila (2013, methodology): “To investigate the function of MBs in the memory-retrieval process of the unimodal conditioning task, we blocked the output of the MBs via expression of the dominant temperature-sensitive shibire (shi) transgene at RT (Kitamoto, 2001) in 247-Gal4.”
Using translational enhancers to increase transgene expression in Drosophila (2012, methodology): “Likewise, effectors of neuronal cell function, such as temperature-sensitive mutants of dynamin encoded by the Shibire gene, can require high levels of protein expression to silence synaptic transmission; for example, the widely used UAS-Shibirets1 stock (Kitamoto III) (31) contains multiple transge”
Dopamine and Octopamine Differentiate between Aversive and Appetitive Olfactory Memories in Drosophila (2003, background): “The line UAS-shi ts1 contains multiple inserts on the X and third chromosomes (Kitamoto, 2001).”
Two independent mushroom body output circuits retrieve the six discrete components of Drosophila aversive memory. (2015, methodology): “The use of the Shibire (Shi) transgenic thermosensitive dominant-negative allele of the dynamin-encoding gene shibire, expressed under the control of the UAS/GAL4 binary system, allows blocking of well-defined neuronal subsets with acute temporal resolution through temperature shifts (Kitamoto, 2001”
Olfactory memory formation in Drosophila: from molecular to systems neuroscience. (2005, background): “At elevated temperatures, neurotransmitter vesicle recycling is inhibited, producing a block in synaptic transmission (Kitamoto 2001).”
Toward a science of computational ethology. (2014, neutral): “, 2008) and shibire (Kitamoto, 2001; Bath et al., 2014) (Table 1).”