Using translational enhancers to increase transgene expression in Drosophila

By B. Pfeiffer, James W. Truman, Gerald M. Rubin. 2012

388 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Immunology, Molecular Biology

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Citations in the corpus

Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014, methodology): “…et al., 2014); pJFRC124-20XUAS-IVS-dTrpA1 (attP18); 10XUAS-dTrpA1 (attP16) (Hamada et al., 2008); pJFRC100-20XUAS-TTS-Shibirets1-p10 (VK00005) (Pfeiffer et al., 2012); UAS-Shi x1 was generated in Thomas Preat's lab by segregating one of the multiple insertions found in the lines described by…”
Two pairs of mushroom body efferent neurons are required for appetitive long-term memory retrieval in Drosophila. (2013, methodology): “, 2011) was obtained from Ann-Shyn Chiang (Tsing Hua University), UAS-IVS-GCaMP3-p10 (inserted in the VK00005 insertion site on chromosome III; also described as pJFRC97; Pfeiffer et al., 2012) was from Barret Pfeiffer and Gerald Rubin (Janelia Farm Research Campus), UAS-ChAT (TRiP collection) from ”
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila (2014, methodology): “We also generated two novel constructs: (i) act-cas9, in which Cas9 is fused to the ubiquitously expressed actin5C (act) promoter and the SV40 3′ UTR, and (ii) UAScas9, which has yeast upstream-activating sequences (UAS), allowing expression under control of the Gal4 transcriptional activator (16), ”
A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, fish, and mice (2018, methodology): “The coding sequence of DA1m-mut was incorporated into pJFRC28 (Pfeiffer et al., 2012) (Addgene plasmid #36431) using Gibson Assembly, and this plasmid was used to generate transgenic flies using PhiC31-mediated site-directed integration into attp40.”
Fast and sensitive GCaMP calcium indicators for imaging neural populations (2021, neutral): “05) and comparable to 8m (84[32-165] ms) and XCaMP-Gf (91[48-155] ms) (Fig.”
Sleep facilitates memory by blocking dopamine neuron mediated forgetting (2015, neutral): “, 2007), TH-lexA (second chromosome) (this study, see below), UAS-shi (PJFRC100-20XUAS-TTS-shibire(ts1)-p10 in attP2, third chromosome) (Pfeiffer et al., 2012), UAS-trpA1 (second chromosome) (Hamada et al.”
A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster (2015, methodology): “DOI: 10.7554/eLife.03868 17 of 24 Research article expression, the 21bp Syn21 sequence (Pfeiffer et al., 2012) was added to the forward primer just upstream of SuperClomeleon.”