Regulation of Clock and NPAS2 DNA Binding by the Redox State of NAD Cofactors

By Jared Rutter, M. Reick, Leeju C. Wu, S. McKnight. Science, 2001

1,046 citations recorded by the source corpus.

Subfields: Cellular and Molecular Neuroscience, Endocrine and Autonomic Systems, Plant Science

View Paper: https://www.semanticscholar.org/paper/5b32d10e974178343c74e588b4131d05acdb72f1

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

1. J. Takahashi
2. U. Albrecht
3. U. Schibler
4. Paolo Sassone-Corsi
5. Cheng Chi Lee
6. Loning Fu
7. J. Bass
8. D. Weaver

In Endocrine and Autonomic Systems

1. J. Takahashi
2. C. H. Ko
3. Paolo Sassone-Corsi
4. U. Schibler
5. U. Albrecht
6. J. Bass
7. Ethan D. Buhr
8. F. Stephan

In Plant Science

1. J. Takahashi
2. C. H. Ko
3. U. Albrecht
4. Paolo Sassone-Corsi
5. Ethan D. Buhr
6. D. Weaver
7. U. Schibler
8. C. Dibner

Citations in the corpus

• The mammalian circadian timing system: organization and coordination of central and peripheral clocks. (2010, background): “Furthermore, at least in vitro, the DNA-binding activity of BMAL1CLOCK is strongly influenced by the ratio of reduced to oxidized NAD cofactors, which are often considered to be readouts of the cellular metabolic state (241).”
• The circadian clock: pacemaker and tumour suppressor (2003, background): “Recently, it was shown that the transcription feedback loops of circadian genes can be regulated by intracellular redox pathway”
• The circadian gene Period2 plays an important role in tumor suppression and DNA damage response in vivo. (2002, background): “…shown recently thatThe University of Texas the neuronal PAS domain protein 2 (NPAS2), a bHLH-1515 Holcombe Boulevard PAS transcription factor that is highly related to CLOCKHouston, Texas 77030 in amino acid sequence, is also a bona fide partner of BMAL1 (Reick et al., 2001; Rutter et al., 2001).”
• The Genetics of Mammalian Circadian Order and Disorder: Implications for Physiology and Disease (2008, background): “For example, in biochemical experiments it can be shown that the reduced forms of the metabolic products NADH and NADPH modulate CLOCK–BMAL1 and NPAS2–BMAL1 heterodimers and enhance their binding to DNA, whereas the oxidized forms NAD and NADP diminish DNA bindin”
• Aerobic glycolysis: meeting the metabolic requirements of cell proliferation. (2011, neutral): “For example, the circadian clock is regulated by NAD(P) cofactors: the reduced forms (NADH and NADPH) activate transcription factors that control gene expression as a function of the light-dark cycle, and the oxidized forms (NAD+ and NADP+) inhibit DNA binding (Rutter et al 2001).”
• Coordination of circadian timing in mammals (2002, background): “The constant binding of CLOCK and BMAL1 to DNA argues against a major role of redox state in modifying the binding of CLOCK–BMAL1 heterodimers to E boxes in vivo”
• NAD+/NADH and NADP+/NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences (2008, neutral): “NADH mediates the activity of the corepressor carboxyl-terminal binding protein—-a transcriptional factor important for cell cycle regulation, development, and transformation (342); and NADH also modulates the activities of Clock:BMAL1 and NPAS2:BMAL1 that are heterodimeric transcription factors con”
• The “Other” Circadian System: Food as a Zeitgeber (2002, background): “…possibility that the heterodimeric transcription factors CLOCK:BMAL1 and NPAS2:BMAL1 may act as redox sensors of nicotinamide dinucleotide because their DNA-binding activity is enhanced in the reduced state and inhibited in the oxidized state in a neuroblastoma cell culture (Rutter et al., 2001).”