Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity

By Ji‐Joon Song, Stephanie K. Smith, Gregory J. Hannon, L. Joshua-Tor. Science, 2004

1,573 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/4b5daddb593dc5685afff40a05c3b8b51f8c274f

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Citations in the corpus

Molecular mechanisms of RNA interference (2013, unknown): “A crystal structure of Argonaute from Pyrococcus furiosus revealed that the PIWI domain adopts an RNase H–like fold, implicating it as the catalytic domain and site of slicer activity (83).”
Perspective: machines for RNAi. (2005, background): “This domain is a structural homolog of RNase H, a Mg-dependent endoribonuclease that cleaves the RNA strand of RNA– DNA hybrids (Liu et al. 2004a; Song et al. 2004).”
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. (2005, unknown): “However, Argonaute2 (Ago2) was recently found to be the catalytic endonuclease of human RISC, and, accordingly, structural studies identified the PIWI domain as the catalytic center that resembles that of RNase H (Liu et al., 2004, Meister et al., 2004, Song et al., 2004; Ma et al., 2005).”
MicroRNA function: multiple mechanisms for a tiny RNA? (2005, background): “…complexes of 15S. Biochemical characterization of these different complexes has led to the identification of a number of protein components, among them Gemin3, Gemin4, vasa intronic gene (VIG), Fragile X mental retardation protein (FMRP), and a staphylococcal nuclease, Tudor-SN (Sontheimer 2005).”
Gene silencing in plants using artificial microRNAs and other small RNAs. (2008, background): “They direct inhibition of target mRNA translation, which is typical for most animal miRNAs, or cleavage of target transcripts opposite position 10/11 of the sRNA, which is typical for siRNAs and plant miRNAs (Lingel et al., 2003; Song et al., 2004).”
The neuronal microRNA system (2006, background): “However, AGO2 can form mRNA cleavage-competent complexes alone in vitro”
Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. (2006, unknown): “The secondary AGO proteins lack key metal-coordinating residues in their RNase H-related PIWI domains, perhaps explaining why siRISC-mediated cleavage activity has not been detected to date in C. elegans.”
microRNAs in action: biogenesis, function and regulation (2023, background): “initial structures of Dicer and Argonaute homologues from protozoan ( Giardia intestinalis ) and archaeal ( Thermus thermophilus and Pyrococcus furiosus ) species 18,48,78–80 .”
The role of PACT in the RNA silencing pathway (2006, background): “…are capable of interacting directly with small RNAs through their PAZ domains (to the 30 end) and through the PIWI and the middle domains (to the 50 end) (Song et al, 2003, 2004; Yan et al, 2003; Lingel et al, 2004; Ma et al, 2004, 2005; Parker et al, 2005; Rivas et al, 2005; Yuan et al, 2005).”
Control of translation and mRNA degradation by miRNAs and siRNAs. (2006, background): “Moreover, mutations in the RNaseH domain of Ago2 abrogate siRNA-mediated cleavage (Liu et al. 2004; Song et al. 2004).”