Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase

By S. Imai, Christopher M. Armstrong, Matt Kaeberlein, L. Guarente. Nature, 2000

3,482 citations recorded by the source corpus.

Subfields: Aging, Geriatrics and Gerontology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4828d9a99b90a5eeaa401626c59361ff97aa8b1c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Aging

1. L. Guarente
2. D. Sinclair
3. E. Verdin
4. M. Haigis
5. Johan Auwerx
6. C. Kenyon
7. Laura Bordone
8. E. Seto

In Geriatrics and Gerontology

1. L. Guarente
2. D. Sinclair
3. Johan Auwerx
4. E. Verdin
5. M. Haigis
6. C. Cantó
7. E. Seto
8. S. Imai

In Molecular Biology

1. L. Guarente
2. E. Verdin
3. Yi Zhang
4. E. Seto
5. Guido Kroemer
6. D. Sinclair
7. M. Haigis
8. D. Reinberg

Citations in the corpus

• NAD+ intermediates: The biology and therapeutic potential of NMN and NR (2017, neutral): “In 2000, Guarente and Imai made the critical discovery that yeast SIR2 (silent information regulator 2) and a mouse ortholog SIRT1 possess NAD+-dependent protein deacetylase activity, fueling new interests in NAD+ biology (Imai et al., 2000).”
• The SMRT and N-CoR Corepressors Are Activating Cofactors for Histone Deacetylase 3 (2001, background): “Gal4SMRT DAD mutants W432A, F440A, F451A, I454A, L458A, V463A, and Y470A were produced using the corresponding mutagenic oligonucleotides and the QuickChange site-directed mutagenesis kit (Stratagene).”
• AMP-Activated Protein Kinase–Deficient Mice Are Resistant to the Metabolic Effects of Resveratrol (2009, background): “One target for treating metabolic disorders is Sirt1 (8), an NAD -dependent protein deacetylase (48).”
• The Role of Sirtuins in Antioxidant and Redox Signaling (2017, background): “However, SIRT2 is also found in the nucleus where it deacetylates H4K16 and takes part in regulating the cell cycle (48).”
• Calorie restriction extends yeast life span by lowering the level of NADH. (2004, background): “2A, X intercept) of Sir2 to be 30 μM, consistent with previous reports (Imai et al. 2000; Tanner et al. 2000).”
• Modulation of mammalian life span by the short isoform of p53. (2004, background): “In addition to its histone deacetylase activity, the mammalian homolog of the yeast Sir2 gene [Sir2 in mouse (Imai et al. 2000) and SIRT1 in human (Frye 1999)] can regulate the activity of p53 (Luo et al.”
• The NAD+-Dependent Deacetylase SIRT1 Modulates CLOCK-Mediated Chromatin Remodeling and Circadian Control (2008, neutral): “We reasoned that CLOCK-mediated acetylation, and thereby transcriptional activation, could be counterbalanced by transcriptional silencing induced by NAD+-dependent HDACs (Imai et al., 2000; Landry et al., 2000).”
• Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration (2002, background): “Sir2 exhibits an NAD-dependent histone deacetylase activity that is conserved among Sir2-family members and is required for chromatin silencing and lifespan extensio”