Derivation of pluripotent epiblast stem cells from mammalian embryos

By I. Brons, L. Smithers, Matthew W. B. Trotter, P. Rugg-Gunn, Bowen Sun, Susana M. Chuva, de Sousa Lopes, S. Howlett, A. Clarkson, L. Åhrlund-Richter and 2 more. Nature, 2007

2,077 citations recorded by the source corpus.

Subfields: Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/34c6a0049f37f4fcd6337d0a4cf7459c01b59119

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Citations in the corpus

Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs (2010, background): “These expression differences between hESCs/hiPSCs and naïve hESCs/hiPSCs are consistent with previously described differences in gene expression between mESCs and mEpiSCs (3, 4).”
Nanog Is the Gateway to the Pluripotent Ground State (2009, neutral): “They are derived from the epithelialized epiblast of post-implantation embryos (Brons et al., 2007; Tesar et al., 2007) or by differentiation of ES cells (Guo et al.”
Somatic cell reprogramming and transitions between pluripotent states: facts, hypotheses, unresolved issues (2010, neutral): “Independent pluripotent lines, called ‘‘EpiSCs,’’ were established by explanting the epiblast from embryonic day 5.5–7.5 of postimplantation mouse embryos in growth conditions supplemented with FGF2 (basic fibroblast growth factor) and Activin (Brons et al., 2007; Tesar et al., 2007).”
Suppression of Erk signalling promotes ground state pluripotency in the mouse embryo (2009, background): “Embryonic stem (ES) cells can be derived from the preimplantation epiblast (Batlle-Morera et al., 2008; Brook and Gardner, 1997; Evans and Kaufman, 1981; Martin, 1981), but have not been derived from the post-implantation epiblast (Brons et al., 2007; Tesar et al., 2007).”
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. (2009, positive): “Pluripotent stem cells have also been derived from the postimplantation egg cylinder stage epiblasts of mouse and rat (Brons et al., 2007; Tesar et al., 2007).”
Senescence impairs successful reprogramming to pluripotent stem cells. (2009, methodology): “derived: epiblast-derived stem (EpiS) cells from post-implantation embryos (Brons et al., 2007; Tesar et al., 2007); embryonic germ (EG) cells from primordial germ cells”
Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition (2008, background): “Fallibility of the Oct4 reporter is not, in fact, surprising given that Oct4 is broadly expressed throughout the pre-pluripotent phase of embryo development and subsequently in the post-implantation egg cylinder [17,18] and derivative epithelialised epiblast stem cells (EpiSCs) [19,20].”
Induced pluripotency: history, mechanisms, and applications. (2010, background): “…derived from post-implantation embryos in the presence of bFGF and activin represent a more advanced or ‘‘primed’’ pluripotent state that exhibits some pluripotency criteria, such as teratoma formation, but fails to contribute efficiently to tissues in mice (Brons et al. 2007; Tesar et al. 2007).”
Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency (2014, neutral): “Because Oct4 is equally expressed betweenmouse ESCs and EpiSCs (Brons et al., 2007; Tesar et al., 2007), this marker does not distinguish a priori between naive and primed states.”
Naive and primed pluripotent states. (2009, methodology): “Indeed, it is now argued that these human cells are analogous to rodent EpiSCs (Brons et al., 2007; Rossant, 2008; Tesar et al., 2007).”
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. (2011, neutral): “It should be noted that hESCs have substantially different properties than mESCs but exhibit characteristics similar to mEpiSCs (Brons et al., 2007; Tesar et al., 2007; Thomson et al., 1998).”