Cargo of Kinesin Identified as Jip Scaffolding Proteins and Associated Signaling Molecules

By K. Verhey, D. Meyer, Renée Deehan, J. Blenis, B. J. Schnapp, Tom A. Rapoport, B. Margolis. Journal of Cell Biology, 2001

632 citations recorded by the source corpus.

The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide repeat (TPR) motifs. Three proteins were found, the c-jun NH2-terminal kinase (JNK)–interacting proteins (JIPs) JIP-1, JIP-2, and JIP-3, which are scaffolding proteins for the JNK signaling pathway. Concentration of JIPs in nerve terminals requires kinesin, as evident from the analysis of JIP COOH-terminal mutants and dominant negative kinesin constructs. Coprecipitation experiments suggest that kinesin carries the JIP scaffolds preloaded with cytoplasmic (dual leucine zipper–bearing kinase) and transmembrane signaling molecules (the Reelin receptor, ApoER2). These results demonstrate a direct interaction between conventional kinesin and a cargo, indicate that motor proteins are linked to their membranous cargo via scaffolding proteins, and support a role for motor proteins in spatial regulation of signal transduction pathways.

Subfields: Cell Biology

View Paper: https://www.semanticscholar.org/paper/337a2b34613d2d6553343f97e49358983c027894

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Citations in the corpus

The axonal transport of mitochondria (2005, background): “, 2000), and two different types of JNK-interacting scaffolding protein (JIP) (Bowman et al., 2000; Verhey et al., 2001; Inomata et al., 2003; Matsuda et al., 2003, Horiuchi et al., 2005).”
Axonal transport of mitochondria to synapses depends on milton, a novel Drosophila protein. (2002, background): “The2000); related jun-interacting proteins (JIPs) that can bind vesicles via ApoER2 (Verhey et al., 2001); and -1 absence of mitochondria and consequent decrease in ATP supply could adversely affect any number of ATP-adaptin, which is associated with post-Golgi vesicles bearing the mannose-6-phospha”
Neuronal polarity: from extracellular signals to intracellular mechanisms (2007, neutral): “c-Jun amino-terminal kinase (JNK)-interacting proteins (JIPs”
Molecular motors and mechanisms of directional transport in neurons (2005, background): “KIF5 also transports other cargoes, including vesicles that contain apolipoprotein E receptor 2 (APOER2)”
Kinesin superfamily motor proteins and intracellular transport (2009, background): “The tetratricopeptide repeat (TPR) domain of KLCs binds to cargos such as JUN N-terminal kinase (JNK)-interacting proteins (JIPs”
Microtubule acetylation promotes kinesin-1 binding and transport. (2006, background): “A direct interaction between the JIP proteins and KLC is required for kinesin-1-dependent transport of JIP1 to the tips of neurites in differentiating neuronal CAD cells [3].”
Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease. (2010, neutral): “Wozniak et al., 2005 KIF2A Vesicles Noda et al., 1995; Morfini et al., 1997 KIF2A Microtubule depolymerizer Homma et al., 2003 KIF3 Fodrin Plasma membrane precursor Takeda et al., 2000 KIF3 Not identified N-cadherin Teng et al., 2005 KIF3 Not identified K v channel Gu et al., 2006 KIF4 Not identifie”
Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress (2002, background): “, 2000), or it could be mediated by adaptor proteins (Morris and Hollenbeck, 1995; Ratner et al., 1998; Setou et al., 2000; Verhey et al., 2001).”