Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures.

By Jayanta Debnath, S. Muthuswamy, J. Brugge. Methods, 2003

2,078 citations recorded by the source corpus.

Subfields: Biotechnology, Immunology and Allergy, Oncology

View Paper: https://www.semanticscholar.org/paper/2edcb78c980686236fa94c2f21d1ed804f938608

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Citations in the corpus

Engineering tumors with 3D scaffolds (2007, methodology): “Culture within this system recreated growth profiles similar to those detected in vivo , whereas tumor cells cultured in standard 3D Matrigel cultur”
Distinct roles of Akt1 and Akt2 in regulating cell migration and epithelial–mesenchymal transition (2005, methodology): “…in vitro model to investigate phenotypic effects that resemble events that take place during breast cancer initiation and progression, such as escape from proliferative suppression, filling of the hollow acinar luminal space, and induction of protrusive, invasive behavior (Debnath et al., 2003a).”
Autophagy mitigates metabolic stress and genome damage in mammary tumorigenesis. (2007, methodology): “3D culture of iMMECs on a reconstituted basement membrane was performed according to the protocol previously described for the immortalized, nontransformed human mammary epithelial cell line MCF-10A (Debnath et al. 2003).”
Modelling glandular epithelial cancers in three-dimensional cultures (2005, background): “3D models of glandular epithelium Under 3D culture conditions, normal epithelial cells typically proliferate and organize into spheroids, commonly called 'acini' (a purely operational term with no anatomical connotation), which are notable for the presence of a centrally-localized, hollow lumen, ”
Microfluidic 3D cell culture: from tools to tissue models. (2015, background): “For example, embedment of cells in an extracellular matrix is associated with more relevant physiological behavior, such as witnessed by apical–basal polarization [1], lumen formation [2], reduced proliferation and increased differentiation [3] and numerous changes in RNA and protein expression”
Normal and neoplastic nonstem cells can spontaneously convert to a stem-like state (2011, background): “1C) and by formation of acinar structures when plated at clonal density on a layer of Matrigel (15).”
Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues (2012, background): “In fact, encapsulating mammary epithelial cells in 3D is not essential for this response, because plating cells on top of basement membrane results in similar morphogenetic events (Debnath et al., 2003).”
Tensional homeostasis and the malignant phenotype. (2005, positive): “To address this, we used BM-crosslinked polyacrylamide (PA) gels (BM gel) with calibrated elastic moduli of 150, 400, 675, 1050, and R5000 Pa. Similar to MECs inside a 3D BM (3D BM; Weaver et al., 2002) or on top of a thick BM overlaid with BM (3D over BM; Debnath et al., 2003), by 16–20 days MECs o”
Mutant p53 Disrupts Mammary Acinar Morphogenesis via the Mevalonate Pathway (2012, positive): “Three-dimensional culture was carried out as previously described (Debnath et al., 2003).”