Microfluidic 3D cell culture: from tools to tissue models.

By V. van Duinen, S. Trietsch, J. Joore, P. Vulto, T. Hankemeier. Current Opinion in Biotechnology, 2015

477 citations recorded by the source corpus.

The transition from 2D to 3D cell culture techniques is an important step in a trend towards better biomimetic tissue models. Microfluidics allows spatial control over fluids in micrometer-sized channels has become a valuable tool to further increase the physiological relevance of 3D cell culture by enabling spatially controlled co-cultures, perfusion flow and spatial control over of signaling gradients. This paper reviews most important developments in microfluidic 3D culture since 2012. Most efforts were exerted in the field of vasculature, both as a tissue on its own and as part of cancer models. We observe that the focus is shifting from tool building to implementation of specific tissue models. The next big challenge for the field is the full validation of these models and subsequently the implementation of these models in drug development pipelines of the pharmaceutical industry and ultimately in personalized medicine applications.

Subfields: Biomedical Engineering, Oncology

View Paper: https://www.semanticscholar.org/paper/879ab9c7001af9f8aa269ca3744fdf016ac9b397

Citations in the corpus

• Biology-inspired Microphysiological System Approaches to Solve the Prediction Dilemma of Substance Testing (2016, unknown): “A recent survey of microfluidic and microengineered 3D cell culture systems conducted by Hankemeier and colleagues (van Duinen et al., 2015) at Leiden University, The Netherlands, found that most tissue modeling efforts published between 2012 and early-2015 in the field of MPS were focused on the…”