Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.

By Jeongwu Lee, S. Kotliarova, Yuri Kotliarov, Aiguo Li, Q. Su, N. Donin, S. Pastorino, B. Purow, Neil Christopher, Wei Zhang and 2 more. Cancer Cell, 2006

2,357 citations recorded by the source corpus.

Subfields: Cancer Research, Genetics, Oncology

View Paper: https://www.semanticscholar.org/paper/2b02f88d0dbb0fbdcaccb3267b23f40f619040ab

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Citations in the corpus

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity. (2019, background): “Traditional in vitro culture models, both monolayer and tumor sphere cultures, can require a substantial amount of time to establish and use exogenous EGF, bFGF, and/or serum to propagate tumor cells over serial passages with clonal expansion, which are not favorable to maintain various cellular sub”
Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity (2008, background): “Using an approach to culture CSCs, which has previously been described for tumorigenic mammaspheres, and gliospheres (9, 17), we now show that spheroid cultures from primary colon carcinoma selects for cells that coexpress all of these CSC markers.”
Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh (2009, background): “Furthermore, Sox2 is expressed in many neural brain tumor cells, particularly in the stem cell componen”
Diffuse glioma growth: a guerilla war (2007, background): “closely mirror the phenotype and genotype of the original tumors than GBM cells cultured in serum [98].”
Multicellular tumor spheroids: an underestimated tool is catching up again. (2010, background): “Human cancers ecently described as having such subpopulations of cells include ot only cancers of the blood but also solid malignancies of brain, reast, pancreas, colon, and head and neck (Cho and Clarke, 2008; alerba et al., 2007; Lee et al., 2006; Ricci-Vitiani et al., 2009).”
microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma. (2008, methodology): “9). Tumor stem cell line 0308 was derived and validated as described previously ( 10 ).”
Donor-Derived Brain Tumor Following Neural Stem Cell Transplantation in an Ataxia Telangiectasia Patient (2009, background): “It is possible that the in vitro expansion of fetal neural stem cells in conditioned medium supplemented with growth factors such as bFGF and EGF promoted transformation [27].”
Phosphorylation of EZH2 activates STAT3 signaling via STAT3 methylation and promotes tumorigenicity of glioblastoma stem-like cells (2013, methodology): “Within hours after surgical removal, tumor specimens were enzymatically dissociated into single cells, following the procedures previously reported (Lee et al., 2006a; Son et al., 2009).”
Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. (2009, background): “Long-term expansion of neurospheres from GBM has, in some cases, been possible in serum-free media, and such cultures are an improvement on ‘‘classic’’ glioma cell lines, such as U87, or other serum-cultured lines, which fail to model accurately the human disease (Galli et al., 2004; Lee et al., 200”
Tumour-initiating cells: challenges and opportunities for anticancer drug discovery (2009, background): “However, these agents are not very effective and the results vary among cellular models, partly because it is difficult to maintain Hedgehog pathway activity in vitro due to differentiation under conventional culture condition”
Cancer stem cells in glioblastoma (2015, background): “Furthermore, high-passage cell lines, which do not offer the ability to accurately represent tumor complexity in vivo (Lee et al. 2006), should not be replaced with cells grown in long-term passage as spheres but ratherwith functionally validatedCSCmodels, as this offers the best opportunity tomore ”