Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.

By S. Pollard, K. Yoshikawa, I. Clarke, D. Danovi, Stefan H. Stricker, R. Russell, J. Bayani, R. Head, Marco Lee, M. Bernstein and 3 more. Cell Stem Cell, 2009

1,027 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Genetics, Oncology

View Paper: https://www.semanticscholar.org/paper/26fce301ddfa67020b17adf7320e19c178db43bc

How researchers see this paper

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In Genetics

1. Jeremy N. Rich
2. J. Lathia
3. Anita B. Hjelmeland
4. F. Doetsch

In Oncology

1. Jeremy N. Rich
2. J. Lathia
3. Anita B. Hjelmeland
4. V. Silva-Vargas
5. Érika Pastrana
6. F. Doetsch
7. P. Dirks

Citations in the corpus

• Integrin alpha 6 regulates glioblastoma stem cells (2010, background): “Laminin is also a key component in culturing relatively pure adherent GSC cultures, suggesting a critical role for the laminin-integrin relationship in GSC maintenance (Fael Al-Mayhani et al., 2009; Pollard et al., 2009).”
• Phosphorylation of EZH2 activates STAT3 signaling via STAT3 methylation and promotes tumorigenicity of glioblastoma stem-like cells (2013, methodology): “…and/or CD15 (Singh et al., 2004; Son et al., 2009) were isolated from surgical specimens of GBM patients or their derivative xenograft tumors and functionally validated in assays of self-renewal and tumor propagation (Joo et al., 2013; Lee et al., 2008; Pastrana et al., 2011; Pollard et al., 2009).”
• Tumour-initiating cells: challenges and opportunities for anticancer drug discovery (2009, background): “, or various serum-free stem cell culture condition”
• Eyes Wide Open: A Critical Review of Sphere-Formation as an Assay For Stem Cells (2011, background): “For example, culture of neural stem cells as cell lines as a monolayer of adherent cells in the presence of EGF and bFGF allows the propagation of a reasonably uniform population of cells with much less differentiation than observed in nonadherent assays (Conti et al., 2005; Pollard et al., 2009).”