Single-cell cloning of colon cancer stem cells reveals a multi-lineage differentiation capacity

By Louis Vermeulen, M. Todaro, F. Mello, M. Sprick, K. Kemper, M. P. Alea, D. Richel, G. Stassi, J. Medema. Proceedings of the National Academy of Sciences of the United States of America, 2008

763 citations recorded by the source corpus.

Subfields: Cancer Research, Oncology

View Paper: https://www.semanticscholar.org/paper/1fe9b9670d4a3ac27425f50bc7ac61e1ba55c2b6

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Citations in the corpus

The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse. (2011, background): “Also, purified cd133 tumor-initiating cells can be expanded in vitro as an undifferentiated population that upon xenotransplantation generates tumors that contain differentiated-like cells (Vermeulen et al., 2008).”
Multicellular tumor spheroids: an underestimated tool is catching up again. (2010, background): “Colospheres from primary human colon cancer cells (Du et al., 2008; Ricci-Vitiani et al., 2007; Todaro et al., 2008; Vermeulen et al., 2008) and mammospheres of putative CSC/TIC from primary breast cancer patients (Grimshaw et al.”
ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays. (2009, background): “Shackleton et al (2006), Quintana et al (2008), Leong et al (2008) and Vermeulen et al (2008) validate the single-hit hypothesis experimentally, by confirming a single input cell in each culture by microscope visualization, before the assay is conducted.”
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis. (2014, background): “, 2011), which maintain the undifferentiated status of the tumorigenic population (Vermeulen et al., 2008).”
Bmi1 is essential in Twist1-induced epithelial–mesenchymal transition (2010, background): “In FaDu cells, there was a significant increase in formation of spheroids, which indicates that the cells are capable of initiating tumour”
The Cancer Stem Cell Niche: How Essential is the Niche in Regulating Stemness of Tumor Cells? (2015, neutral): “…only cells instigating or maintaining metastasis, the CSC-generated hierarchy of stem-like and differentiated tumor cells is able to initiate metastatic growth and is also seen in late-stage cancers and atmetastatic sites (Dalerba et al., 2011; Merlos-Suárez et al., 2011; Vermeulen et al., 2008).”
A chromatin-mediated reversible drug tolerant state in cancer cell subpopulations (2010, background): “DTPs were also highly enriched (relative to parental PC9 cells) for expression of CD24, another CSC marker in some settings (Vermeulen et al., 2008), whereas another CSC marker, CD44, was equally represented”
Wnt activity defines colon cancer stem cells and is regulated by the microenvironment (2010, background): “Results Colon cancer spheroidal cultures have heterogeneous Wnt activity levels Primary spheroidal cultures of colon cancer cells consist of cells expressing CSC markers and on injection induce a tumour that closely resembles the original human malignanc”