Real-time PCR in virology.

By I. Mackay, K. Arden, Andreas Nitsche. Nucleic Acids Research, 2002

1,405 citations recorded by the source corpus.

Subfields: Agronomy and Crop Science, Epidemiology, Genetics

View Paper: https://www.semanticscholar.org/paper/248983319e84ed7ebe127072af74760c777fa1c2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Agronomy and Crop Science

1. I. Mackay

In Epidemiology

1. I. Mackay
2. Andreas Nitsche
3. Stephen A. Bustin
4. Aleksandar Radonic
5. Stefanie Thulke
6. W. Siegert
7. O. Landt

In Genetics

1. I. Mackay
2. Andreas Nitsche
3. Stephen A. Bustin
4. Franco Nigro
5. Aleksandar Radonic
6. Stefanie Thulke
7. A. Ippolito
8. L. Schena

Citations in the corpus

• Guideline to reference gene selection for quantitative real-time PCR. (2004, background): “Changes in mRNA transcription levels are crucial during developmental processes, therapeutic drug treatment of disease, tumorigenesis, and for the diagnosis and quantification of viral disease [1].”
• PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings (2004, neutral): “67 Each has a proven cost-saving role in clinical practice, including detection of otherwise difficult to diagnose infections, and a newly realised capacity to monitor progression of disease and response to therapy, vital to the management of chronic infectious diseases.”
• The power of real-time PCR. (2005, background): “Mutation analyses using melting curves enable individual and epidemiological studies of viral coinfections or quasispecies (31).”
• Real-time PCR in the microbiology laboratory. (2004, methodology): “Fluorescence data generated by real-time PCR assays are generally collected from PCR cycles that occur within the linear amplification portion of the reaction, where conditions are optimal and the fluorescence accumulates in proportion to the amplicon [52] (Fig.”
• Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases (2008, methodology): “The real-time assays have many advantages over conventional PCR methods, including rapidity, quantitative measurement, lower contamination rate, higher sensitivity, higher specificity and easy standardisation [1].”
• Real-Time PCR (2005, background): “Other uses include the assessment of viral gene therapy vectors before their use in clinical trials and the study of new and emerging viruses and clinical symptoms experienced by patients.([24]) A major benefit lies in the rapidity with which results can be obtained.”
• Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi (2004, methodology): “These chemistries can be grouped into amplicon sequence non-specific and sequence specific methods (Mackay et al., 2002).”
• Real-time PCR for detection and quantification of fungal and oomycete tomato pathogens in plant and soil samples (2006, methodology): “Since the development of the exponential phase of the reaction is monitored, real-time PCR allows accurate template quantification [10].”
• Reference genes identified in the silkworm Bombyx mori during metamorphism based on oligonucleotide microarray and confirmed by qRT‐PCR (2008, background): “When organisms experience physiological events such as developmental meta- morphosis or viral aggression, the changes in mRNA transcription levels are crucial (Mackay et al., 2002).”
• Real-time turbidimetry of LAMP reaction for quantifying template DNA. (2004, methodology): “Furthermore, the real-time PCR method is likely to be regarded as an essential method in quantitative genetic tests for infectious diseases because of the development of new technologies such as the Taqman probe method and the Molecular Beacon method, which compensate for the shortcoming of low spec”
• Modelling the PCR amplification process by a size-dependent branching process and estimation of the efficiency (2004, background): “Quantitative analysis is now a major use of PCR and has many applications in virology [13], gene-expression studies [25] or pathogenic bacteria detection [9].”
• Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing. (2006, background): “Reactions targeting smaller amplicons have been shown to be less vulnerable to inhibitors of the amplification reaction (Mackay et al., 2002).”