Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing.

By Wenbin Li, J. Hartung, L. Levy. Journal of Microbiological Methods, 2006

812 citations recorded by the source corpus.

Subfields: Horticulture, Plant Science

View Paper: https://www.semanticscholar.org/paper/29ebd84d051c6747be0cb42afb0c4c19eef35eb3

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Horticulture

1. J. Graham
2. D. Hall
3. E. Ammar
4. K. Bowman
5. Ute Albrecht
6. T. McCollum
7. B. Wood
8. Mike Irey

In Plant Science

1. D. Hall
2. E. Ammar
3. Lukasz L. Stelinski
4. F. Engelmann
5. Qiaochun Wang
6. J. Graham
7. B. Panis
8. M. Lambardi

Citations in the corpus

• Detection and Relative Titer of Candidatus Liberibacter asiaticus in the Salivary Glands and Alimentary Canal of Diaphorina citri (Hemiptera: Psyllidae) Vector of Citrus Huanglongbing Disease (2011, methodology): “The Q-PCR reactions were run on DNA isolated from speciÞed body parts ofD. citri by using the APHIS-recommended TaqMan protocol and speciÞed primers and probe (Li et al. 2006).”
• Quantification of viable Candidatus Liberibacter asiaticus in hosts using quantitative PCR with the aid of ethidium monoazide (EMA) (2009, methodology): “It has been speculated that the HLB pathogen is present in the citrus phloem at low titer ( Li et al. 2006 ).”
• Changes in carbohydrate metabolism in Citrus sinensis infected with ‘Candidatus Liberibacter asiaticus’ (2010, positive): “asiaticus’, the bacterium associated with HLB, was confirmed by quantitative real-time PCR as described by Li et al. (2006). Yellowing and blotchy mottle symptoms began to show 17 weeks after inoculation (WAI).”
• Tolerance of trifoliate citrus rootstock hybrids to Candidatus Liberibacter asiaticus (2012, methodology): “For detection f Las, real-time PCR assays were performed using primers HLBas nd HLBr and probe HLBp developed by Li et al. (2006).”
• Status of Huanglongbing (HLB) outbreaks in Florida, California and Texas (2020, methodology): “99) for determination of infection (Li et al. 2007).”
• Inconsequential effect of nutritional treatments on huanglongbing control, fruit quality, bacterial titer and disease progress (2012, methodology): “All trees included in the experiment were confirmed to be Las-positive via a real-time quantitative polymerase chain reaction (qPCR) assay (Li et al., 2006).”
• Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation (2009, background): “Phytoplasmas and the obligate bacterial parasites inhabit the plant vasculature, invade only tissues that support their movement and are unevenly distributed (Li et al., 2006; Hogenhout et al., 2008).”
• Plant pathogen-induced volatiles attract parasitoids to increase parasitism of an insect vector (2014, methodology): “Lasspecific 16S rDNA from plant extracts was amplified using probeprimer sets targeting internal control sequences specific to plant [cytochrome oxidase] gene regions (Li et al., 2006; Pelz-Stelinski et al., 2010).”
• Visible-near infrared spectroscopy for detection of Huanglongbing in citrus orchards (2011, methodology): “Although laboratory techniques, such as polymerase chain reaction (PCR) provide accurate detection of HLB (Teixeira et al., 2005; Li et al., 2006, 2009; Lacava et al., 2006), faster and lower cost field-based methods are needed for the rapid detection of this disease.”