Real-time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi

By L. Schena, Franco Nigro, A. Ippolito, D. Gallitelli. European journal of plant pathology, 2004

347 citations recorded by the source corpus.

Subfields: Cell Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/9f8f9973146804266f37a1c4a8e49906c509246c

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cell Biology

1. B. Schulz
2. L. Schena
3. C. Boyle
4. G. Berg
5. A. Ippolito
6. D. Cooke
7. M. Mazzola
8. A. McLeod

In Plant Science

1. B. Schulz
2. L. Schena
3. A. Ippolito
4. C. Boyle
5. D. Cooke
6. G. Berg
7. B. Thomma
8. A. McLeod

Citations in the corpus

• Development and application of a PCR‐based ‘molecular tool box’ for the identification of Phytophthora species damaging forests and natural ecosystems (2007, background): “However, ITS sequences are not always sufficiently variable to separate closely related taxa (Kroon et al ., 2004; Schena & Cooke, 2006; Schena et al., 2006) and the development of SCAR primers is very laborious (Schena et al., 2004).”
• Real-time PCR for detection and quantification of fungal and oomycete tomato pathogens in plant and soil samples (2006, methodology): “However, with the exception of a few studies [12–14] the interaction between a single plant species and only one or two related pathogens has been reported to date.”
• Early detection of Botrytis cinerea latent infections as a tool to improve postharvest quality of table grapes (2012, background): “gel electrophoresis), it is possible to develop detection protocols that are quantitative, highly sensitive, specific, reliable, and suitable for high throughput analysis (Schena et al., 2004).”
• The endophytic continuum. (2005, methodology): “Real-time PCR (Schena et al. 2004) is presumably the most accurate method for quantifying fungal colonisation within the host and has been successfully employed, for example by Winton et al.”
• Identification and quantification of Fusarium oxysporum in planta and soil by means of an improved specific and quantitative PCR assay (2010, neutral): “) (Schaad et al., 2003; Schena et al., 2004) that are more specific but with an increased cost of analysis.”
• Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance (2012, background): “alboatrum (Schena et al., 2004) and to distinguish pathogenic and non-pathogenic Fusarium oxysporum in tomato (Validov et al.”
• Isolation Procedures for Endophytic Microorganisms (2006, methodology): “Real-time PCR (Vaitilingom et al. 1998; Schena et al. 2004 ) is presumably the most accurate method for quantifying microbial colonisation within the host and has been successfully employed, e.g.”
• Characterization of fungi (Fusarium and Rhizoctonia) and oomycetes (Phytophthora and Pythium) associated with apple orchards in South Africa (2011, background): “Quantitative PCR (qPCR) in particular, has become a powerful tool for detection and quantification of different plant pathogenic microorganisms (Schena et al. 2004), since quantification based on culturing techniques is considered relatively inaccurate and in some cases even unreliable (Goud and…”