Real-Time PCR

By Stephen A. Bustin. 2005

850 citations recorded by the source corpus.

Subfields: Biomedical Engineering, Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8d73f326d85e17b4c17608921cb031ad4c20f4d2

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In Biomedical Engineering

In Ecology

In Molecular Biology

Citations in the corpus

Global Dissemination of Carbapenemase-Producing Klebsiella pneumoniae: Epidemiology, Genetic Context, Treatment Options, and Detection Methods (2016, methodology): “As with KPCs and NDMs, various detection methods were developed to identify OXA-48-like carbapenemases (Tsakris et al., 2010; Giske et al., 2011; Girlich et al., 2013; Naas et al., 2013; Lee et al., 2015c).”
Optimization of DNA extraction for quantitative marine bacterioplankton community analysis (2004, methodology): “…as real-time PCR or hybridization aiming at DNA or RNA have the potential to quantify population dynamics through the presence or expression of specific genes with a high sensitivity and resolution (Rehnstam et al. 1993; Guschin et al. 1997; Pinhassi et al. 1997; Roth 2002; Wawrik et al. 2002).”
High-resolution genotyping by amplicon melting analysis using LCGreen. (2003, methodology): “These dye concentrations are near the maximum PCR-compatible concentrations, as determined by real-time PCR analysis using the second-derivative maximum method (3, 16).”
Choosing natural enemies for conservation biological control: use of the prey detectability half‐life to rank key predators of Colorado potato beetle (2010, neutral): “Provided the stage consumed can be determined, the number of individuals consumed might be estimated by RT-PCR (Nejtstgaard et al., 2007; Zhang et al., 2007; Weber & Lundgren, 2009).”
Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons. (2004, background): “Most of these techniques can be performed after amplification is complete, even though they are often associated with real-time PCR (33-37).”
Evaluation of coffee reference genes for relative expression studies by quantitative real-time RT-PCR (2009, background): “The high sensitivity and specificity of qPCR analysis, even when limited amounts of RNA are available, have boosted its use among plant biologists (Gachon et al. 2004).”