Wnt proteins are lipid-modified and can act as stem cell growth factors

By K. Willert, Jeffrey Brown, E. Danenberg, A. Duncan, Irving L. Weissman, T. Reya, John R. Yates III, R. Nusse. 2003

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In Molecular Biology

Citations in the corpus

A role for Wnt signalling in self-renewal of haematopoietic stem cells (2003, background): “The expansion of HSCs owing to activated β-catenin probably reflects upstream Wnt signals, as we have demonstrated recently that purified Wnt3a causes self-renewal in both BCL-2 transgeni”
Differentiation and persistence of memory CD8+ T cells depend on T cell factor 1 (2010, background): “We stimulated naive CD8+ T cells with Wnt3a, which has been shown to promote proliferation of hematopoietic stem cells via accumulation of b-catenin (Willert et al., 2003).”
Wnt signaling in development and tissue homeostasis (2018, background): “This is an essential step in Wnt protein secretion (Herr and Basler, 2012) and introduces a modification that is required for Wnt activity (Janda et al., 2012; Proffitt and Virshup, 2012; Willert et al., 2003).”
LDL receptor-related proteins 5 and 6 in Wnt/β-catenin signaling: Arrows point the way (2004, background): “Nevertheless, recent success in purification of the first Wnt protein, mouse Wnt3a, may help to pave the way for comprehensive biochemical studies of Wnt-Fz interactions in vitro (Willert et al., 2003).”
Wnt Signaling Requires the Sequestration of Glycogen Synthase Kinase 3 inside Multivesicular Endosomes (2010, positive): “L-cell control conditioned medium and Wnt3a conditioned medium were harvested according to the ATCC protocol (Willert et al., 2003).”
Clathrin- and non-clathrin-mediated endocytic regulation of cell signalling (2005, background): “Although the analysis of Wnt-receptor trafficking is in its infancy, it is nonetheless interesting that Wnt ligands are highly insoluble, probably due to modification by palmitoylatio”
Structural basis of WLS/Evi-mediated Wnt transport and secretion (2020, background): “When Wnt covalent O-acylation was first identified, speculation centered on a membrane-tethering or lipoprotein-binding role for the fatty acid, similar to the role the far more common S-palmitoylation plays in diverse proteins (Panáková et al., 2005; Smotrys and Linder, 2004; Takada et al., 2006; W”
Wnts and Hedgehogs: lipid-modified proteins and similarities in signaling mechanisms at the cell surface (2003, background): “It has been proposed (Tamai et al., 2000), but not always confirmed (Wu and Nusse, 2002), that Wnt molecules can also bind to LRP and form a trimeric complex with a Frizzled.”