Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon

By P. Olsvik, K. Lie, A. Jordal, T. Nilsen, I. Hordvik. BMC Molecular Biology, 2005

642 citations recorded by the source corpus.

BackgroundSalmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking.ResultsThe stability of six potential reference genes was examined in eight tissues of Atlantic salmon (Salmo salar), to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. The relative transcription levels of genes encoding 18S rRNA, S20 ribosomal protein, β-actin, glyceraldehyde-3P-dehydrogenase (GAPDH), and two paralog genes encoding elongation factor 1A (EF1AA and EF1AB) were quantified in gills, liver, head kidney, spleen, thymus, brain, muscle, and posterior intestine in six untreated adult fish, in addition to a group of individuals that went through smoltification. Based on calculations performed with the geNorm VBA applet, which determines the most stable genes from a set of tested genes in a given cDNA sample, the ranking of the examined genes in adult Atlantic salmon was EF1AB>EF1AA>β-actin>18S rRNA>S20>GAPDH. When the same calculations were done on a total of 24 individuals from four stages in the smoltification process (presmolt, smolt, smoltified seawater and desmoltified freshwater), the gene ranking was EF1AB>EF1AA>S20>β-actin>18S rRNA>GAPDH.ConclusionOverall, this work suggests that the EF1AA and EF1AB genes can be useful as reference genes in qRT-PCR examination of gene expression in the Atlantic salmon.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/6ff185c1419792ea6d4edd291f5f5e86f788c631

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. W. McNabb

In Molecular Biology

1. L. Valente
2. J. Fernandes
3. I. Johnston
4. D. Lai
5. S. McCormick
6. A. Dodd
7. S. Stefansson
8. Luís E. C. Conceição

Citations in the corpus

• Validation of Zebrafish (Danio rerio) Reference Genes for Quantitative Real-time RT-PCR Normalization (2007, background): “[9] validated the EF1AA and EF1AB genes as suitable reference genes for transcript studies of tissues in the same fish species, and Ingerslev et al.”
• Differential expression of gill Na+,K+-ATPaseα - and β-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar (2007, methodology): “1 for all target genes (NKA- 1a, NKA- 1b, NKA- 1c, NKA- 3, NKA- 1 and NKCC) in relation to elongation factor 1A (EF1AA) (Olsvik et al., 2005).”
• What determines growth potential and juvenile quality of farmed fish species (2013, background): “2008), Atlantic salmon (Olsvik et al. 2005), European sea bass (Mitter et al.”