Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis1[w]

By Tomasz Czechowski, Mark Stitt, Thomas Altmann, Michael K. Udvardi, W. Scheible. Plant Physiology, 2005

1,665 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/8ad2ce7f45422c3b33b4a9d7246546b366fe13d7

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In Molecular Biology

1. Kazuo Shinozaki
2. M. B. Bitonti
3. Maria Greco
4. A. Chiappetta
5. L. Bruno
6. Kazuki Saito
7. Jiani Qi
8. Xiuyun Zhao

Citations in the corpus

• Reference Gene Selection for Real-Time Quantitative Polymerase Chain Reaction of mRNA Transcript Levels in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (2010, background): “Furthermore, Clathrin was identified as a novel reference gene in A. thaliana through microarray experiments (Czechowski et al. 2005), but its Cp value fluctuated widely in our sample sets.”
• The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. (2008, background): “At4g34270 and At4g33380 are two genes found to be stably expressed in Arabidopsis (Czechowski et al., 2005).”
• LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana (2008, methodology): “Data were normalized to the mean CT of three reference genes (At4g27960, At5g46630, and At4g34270) that were found to be stably expressed across various tissues and conditions [ 118 ].”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, positive): “To avoid amplification from contaminating genomic DNA, primers were designed to span predicted introns (Czechowski et al., 2005).”
• The AtGenExpress hormone and chemical treatment data set: experimental design, data evaluation, model data analysis and data access. (2008, background): “Czechowski et al. (2005) identified genes with very stable expression patterns as reference genes for gene expression studies.”
• Association of a Soybean Raffinose Synthase Gene with Low Raffinose and Stachyose Seed Phenotype (2008, result): “Th e results indicated that the putative raffi nose synthase genes have similar transcript levels in all tissues examined as compared to the housekeeping gene elongation factor 1α (Czechowski et al., 2005).”