Standard methods for molecular research in Apis mellifera

By Jay D. Evans, Ryan S Schwarz, Yanping Chen, G. Budge, R. Cornman, P. Rúa, J. Miranda, S. Forêt, L. Foster, L. Gauthier and 13 more. 2013

192 citations recorded by the source corpus.

Subfields: Ecology, Evolution, Behavior and Systematics, Genetics, Insect Science

View Paper: https://www.semanticscholar.org/paper/92635f6bd3361fe2ef02cf83b8014fe5fe86335f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology, Evolution, Behavior and Systematics

1. P. Neumann
2. J. Ellis
3. J. Pettis
4. R. Paxton
5. Per Kryger
6. M. Schäfer
7. V. Dietemann
8. Philipp Engel

In Genetics

1. P. Neumann
2. J. Ellis
3. J. Pettis
4. M. Schäfer
5. Jay D. Evans
6. R. Paxton
7. Per Kryger
8. N. Moran

In Insect Science

1. P. Neumann
2. J. Ellis
3. J. Pettis
4. M. Schäfer
5. Jay D. Evans
6. J. M. Sforcin
7. C. Pirk
8. M. Meixner

Citations in the corpus

• The COLOSS BEEBOOK Volume II, Standard methods for Apis mellifera pest and pathogen research: Introduction (2013, methodology): “The general instructions to use microsatellites are given in the molecular methods paper (Evans et al., 2013), whereas the specific method appropriate for subspecies identification is described in the paper on ecotypes and subspecies identification (Meixner et al., 2013).”
• Standard methods for rearing and selection of Apis mellifera queens (2013, methodology): “Microsatellite analysis and other molecular methods can be used to identify the individual origin of drones or its semen from certain colonies (see the BEEBOOK papers on molecular techniques (Evans et al., 2013), and miscellaneous research methods (Human et al., 2013)).”
• Standard methods for characterising subspecies and ecotypes of Apis mellifera (2013, methodology): “Therefore, a full and accurate identification requires employment of both mtDNA and nuclear markers (see also the BEEBOOK paper on molecular methods (Evans et al., 2013)). extensively used in studies about the spread of Africanized honey bees, or in studies on differentiation of European lineages…”
• Standard methods for research on Apis mellifera gut symbionts (2013, background): “Its use in honey bee research is discussed further in Evans et al. (2013).”
• Standard methods for small hive beetle research (2013, methodology): “The methods for these analyses will be identical to those used previously for honey bees and other insects, and these methods are described in the BEEBOOK paper on molecular methods (Evans et al., 2013).”
• Standard methods for Apis mellifera propolis research (2016, methodology): “For PCR methods to identify A. apis, please refer to Jensen et al. (2013).”
• Standard methods for Nosema research (2013, methodology): “Set up a standard curve using serial dilutions of recombinant target DNA fragments ranging from 10-2 to 10-8 and include them as quantification standards in every PCR run (see also the BEEBOOK paper on molecular methods (Evans et al., 2013)).”
• Standard methods for virus research in Apis mellifera (2013, methodology): “There are several alternatives for different purposes and samples sizes (see the BEEBOOK paper on molecular techniques; Evans et al., 2013), including the ‘dry shipper’ which can hold up to 1300 2-ml cryo-tubes below freezing for up to 3 weeks and is approved for international air-shipment.”
• The COLOSS BEEBOOK Volume I, Standard methods for Apis mellifera research: Introduction (2013, methodology): “The general instructions to use microsatellites are given in the molecular methods paper (Evans et al., 2013), whereas the specific method appropriate for subspecies identification is described in the paper on ecotypes and subspecies identification (Meixner et al., 2013).”
• Standard methods for European foulbrood research (2013, methodology): “…possible to identify the presence of M. plutonius in the absence of disease symptoms by collecting bulk samples of 100 larvae, taken at random from across the brood nest and subjecting the samples to qPCR (Budge et al., 2010; see also the molecular methods paper of the BEEBOOK (Evans et al., 2013).”