Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing.

By Xiaofeng Cao, Steven E. Jacobsen. Current Biology, 2002

779 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/16949db2e3c523f31ab44cf74cd52c2d9ab68612

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Plant Science

Citations in the corpus

Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning (2006, neutral): “CNG and asymmetric methylation are maintained by the DRM2 and CMT3 methyltransferases, and DRM2 controls de novo methylation in all sequence context”
Gardening the genome: DNA methylation in Arabidopsis thaliana (2005, background): “Arabidopsis thaliana drm1 drm2 double mutants lack every type of de novo DNA methylation tested, including methylation of transformed tandem repeats, transcribed inverted repeats and DNA methylation induced by the transgenic SUPERMAN inverted repeat called clk – st”
The role of RNA interference in heterochromatic silencing (2004, background): “Double mutants of the redundant de novo non-CG DNA methyltransferases domains rearranged methylase 1 and domains rearranged methylase 2 ( drm1 and drm2 ) ( Box 2 ) also fail to silence introduced transgenes: thus DRM1 and DRM2 are likely to establish DNA methylation in this cas”
Transposable elements and the epigenetic regulation of the genome (2007, background): “thaliana requires DRM2 and CMT3 , but transgenes and TEs are only selectively reactivated in these mutants if the symmetrical CpG maintenance methyltransferase MET1 (the homologue of Dnmt1 ) is still presen”
Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis. (2006, methodology): “Bisulfite Sequencing Genomic bisulfite sequencing was performed as previously described (Cao and Jacobsen, 2002).”
DNA methylation mediated by a microRNA pathway. (2010, background): “LmiRNAs bound to AGO4 proteins interact with nascent transcripts transcribed from their own loci or target genes, thereby recruiting de novo cytosine methyltransferase DRM2 to methylate the adjacent DNA. lmiRNAs with a 50 U were sorted into AGO4b but not into AGO1 clade proteins (Figure 3; Table S3)”
Plant nuclear RNA polymerase IV mediates siRNA and DNA methylation-dependent heterochromatin formation. (2005, background): “Importantly, CG, CNG, and CNN de novo methylation is accomplished by DRM methyltransferase activity (Cao et al., 2003; Cao and Jacobsen, 2002).”
Highly integrated single-base resolution maps of the epigenome in Arabidopsis. (2008, positive): “DNA methylation is established in all contexts by DRM1/2, homologs of the mammalian DNMT3a/b de novo DNA methyltransferases (Cao et al., 2003; Cao and Jacobsen, 2002).”