Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits

By C. Merrifield, M. Feldman, Lei Wan, W. Almers. Nature Cell Biology, 2002

722 citations recorded by the source corpus.

Subfields: Atomic and Molecular Physics, and Optics, Cell Biology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/15460d824abcace05f8680e40c0c3f66b183bb68

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Citations in the corpus

The Ark1/Prk1 family of protein kinases (2003, background): “Analysis of the in vivo substrate specificities of these molecules, coupled with improved microscopy techniques that allow local actin dynamics to be imaged (Merrifield et al., 2002), should provide important insights into these issues.”
Dynasore, a cell-permeable inhibitor of dynamin. (2006, background): “colocalizes with spots containing AP-2 (Figure 4A, vehicle) or clathrin (Figure S4A) (Damke et al., 1994; Ehrlich et al., 2004; Merrifield et al., 2002 ), visualized here by the fluorescence of s2-adaptin-EGFP (or EGFP-LCa) and by labeling with an antibody specific for dynamin2.”
A High Precision Survey of the Molecular Dynamics of Mammalian Clathrin-Mediated Endocytosis (2011, methodology): “Sequential Recruitment of Positive and Negative Regulators of Actin Polymerization It is generally accepted that actin polymerization plays a role in some (but not all, see [52,53]) forms of CME [9,11,18,44,46, 47,50,54].”
Actin assembly and endocytosis: from yeast to mammals. (2003, background): “Biochemical studies and imaging of clathrin-DsRed in live cells suggest that uncoating occurs soon after CCVs pinch off from the plasma membrane (Gaidarov et al. 1999, Merrifield et al. 2002).”
Harnessing actin dynamics for clathrin-mediated endocytosis (2006, background): “The dynamic connection between the actin cytoskeleton and sites of clathrin-mediated endocytosis in living cells was first shown in cultured mammalian cell”
A modular design for the clathrin- and actin-mediated endocytosis machinery. (2005, positive): “Similar to mammalian cells, in yeast, clathrin localizes to cortical patches that precede actin bursts (Merrifield et al., 2002; Newpher et al., 2005).”
Derailed endocytosis: an emerging feature of cancer (2008, methodology): “By using total internal reflection fluorescence microscopy and fluorescently tagged actin, Merrifield and colleagues first observed in endocytic loci 'actin bursts' that preceded internalization of the endocytic coat and coincided with transient recruitment of dynamin”
Regulated portals of entry into the cell (2003, background): “Recent kinetic data show that actin assembly is spatially and temporally coordinated with the recruitment of dynamin to the necks of coated pits, and with the release of CCVs into the cytoso”
Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins. (2005, background): “Since growing evidence suggests an involvement of actin in the fission reaction of endocytosis (Kaksonen et al., 2003; Merrifield et al., 2002, 2005; Yarar et al., 2005), the actin cytoskeleton may be an effector of dynamin in this reaction.”
Feedback regulation of EGFR signalling: decision making by early and delayed loops (2011, background): “The multi-component and modular structure of the endocytosis machinery provides multiple interfaces for signal transduction; the actin cytoskeleto”