Gene targeting by homologous recombination in Drosophila.

By Y. Rong, K. Golic. Science, 2000

610 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/091016b09dfdfab00d433265d7b944342cc806f6

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Citations in the corpus

Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. (2004, background): “…cule by expression of FLP and made linear by the expresSpradling et al. 1999), position effects can strongly sion of I-SceI, increasing the targeted recombination influence gene expression, complicating the pheno- frequency (Rong and Golic 2000, 2001; Rong et al. typic analysis (Levis et al. 1985).”
A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster. (2001, background): “Additionally, because it is now practical to make targeted mutants in Drosophila (Rong and Golic 2000), it should soon be feasible to generate loss-of-function mutants in any of these genes.”
Site-specific selfish genes as tools for the control and genetic engineering of natural populations (2003, background): “Encouragingly, a yeast HEG has recently been shown to be active in stimulating recombinational repair in D. melanogaster (Bellaiche et al. 1999; Rong & Golic 2000; Rong et al. 2002).”
Stimulation of Homologous Recombination through Targeted Cleavage by Chimeric Nucleases (2001, background): “In the former case, target cleavage might be combined with the recently described procedures for producing linear donor DNA in vivo (45) to achieve maximum efficiency.”
Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila. (2009, neutral): “, 1994), Df(2L)M36F-S6, Df(3R)p13 (Bloomington Drosophila Stock Center), 70FLP,70I-SceI/Cyo and 70FLP (Rong and Golic, 2000), UAS-mCD8:GFP (Lee and Luo, 1999), OR35a-GAL4 (Fishilevich and Vosshall, 2005).”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, background): “The result of ends-in gene targeting is a local duplication at the targeting site, due to the integration of the entire targeting vector (Rong and Golic, 2000, 2001).”
Highly Efficient Targeted Mutagenesis of Drosophila with the CRISPR/Cas9 System (2013, background): “, 1991), increasing efficiency as compared to current methods (Maggert et al., 2008; Rong and Golic, 2000).”
Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease (2013, background): “…cleavage of Drosophila genes To test whether the CRISPR RNA/Cas9 system could induce site-specific DSBs in Drosophila, we targeted the yellow gene, which is on the X chromosome and commonly used for genome engineering studies (Rong and Golic 2000; Bibikova et al. 2002; Liu et al. 2012).”
Flies, genes, and learning. (2001, background): “Obtaining direct gene knockouts in flies is now possible (Rong & Golic 2000) although it is methodologically tricky at present, but making and introducing dominant-negative gene products is easy.”
Post-transcriptional gene silencing by double-stranded RNA (2001, background): “elegans , the impossibility) of generating targeted null mutations analogous to mouse knockout”
Drosophila Aurora B Kinase Is Required for Histone H3 Phosphorylation and Condensin Recruitment during Chromosome Condensation and to Organize the Central Spindle during Cytokinesis (2001, background): “The proximity of other genes made the prospect of generating such mutations by classical genetic means somewhat daunting and although a model system for the targeted disruption of genes has recently been developed (Rong and Golic, 2000), this is also a time-consuming and not yet widely used procedur”
Genetic dissection of neural circuits. (2008, neutral): “This can be achieved in mice and flies (Capecchi, 1989; Rong and Golic, 2000).”